牡丹籽油对小鼠急性肝损伤的保护作用  

Protective effect of peony seed oil on acute liver injury in mice

作　　者：翟文婷[1] 朱献标[1] 李艳丽[1] 杨印军 许卉[1] 

机构地区：[1]烟台大学药学院,山东烟台264005

出　　处：《中国油脂》2013年第11期43-45,共3页China Oils and Fats

基　　金：烟台大学大学生科技创新基金项目(111403);烟台大学实验室开放基金项目(2011-2012学年第二学期)

摘　　要：为研究牡丹籽油(PEO)对小鼠急性肝损伤的保护作用,实验采用48只雄性昆明种小鼠,随机分为正常组、模型组、PEO组(PEO-L、PEO-M、PEO-H)和阳性对照组(丹酚酸A,SAA)。其中,PEO组和SAA组分别按1.3、4.0、12.0 g/(kg·d)和7.5 mg/(kg·d)剂量灌胃给予PEO和SAA的1%CMC混悬液,正常组和模型组灌胃给予相同体积的1%CMC溶液,连续给药4周。末次给药24 h后,经腹腔注射给予CCl420 mg/kg(正常组除外)。16 h后采集各组动物血清及肝脏组织样本,测定各项生化指标。结果表明:与模型组相比,PEO-M组的肝指数显著降低(P<0.05);PEO各组均可极显著降低血清GOT、GPT水平(P<0.01),其中PEO-H组和PEO-M组的作用极显著强于SAA组(P<0.01);PEO-M组可显著提高肝脏SOD水平(P<0.05),并能极显著降低肝脏MDA,提高GSH-PX水平(P<0.01),作用强度与SAA相当(P>0.05)。因此,适量摄入PEO对小鼠急性肝损伤有显著的保护作用。The protective effect of peony seed oil （PEO） on acute liver injury in mice was investigated. Forty -eight Kunming mice were randomly divided into six grups ：normal control, model, positive control （salvianolic acid A,SAA） and PEO groups（ PEO -H,PEO -M and PEO -L）, The mice were daily ad- ministered with 1% CMC suspension of PEO and SAA at the dosage of 1.3,4.0,12.0 g/（ kg ·d） and 7.5 mg/（kg·d） for the groups of PEO- L,PEO -M,PEO -H and SAA respectively,while the same volume of 1% CMC were given to normal control and model groups for four weeks. After twenty - four hours of the last administration, the mice （ except the normal control group） were injected intraperitoneal- ly with CCI4 （20 mg/kg）. All the mice were sacrificed sixt~：en hours later, and serum and liver of the mice were collected for biochemical indexes determination. The results showed that comparing with the model group, the liver index in PEO - M group decreased sigrLificantly （P 〈 0.05 ）. The serum GOT and GPT levels in PEO groups decreased significantly （ P 〈 0.01 ）, and the decreasing effects in PEO - M and PEO - H groups were superior than that in SAA group （ P 〈 0.01 ） ; SOD level increased （ P 〈 0.05 ）, MDA reduced and GSH - PX level increased （ P 〈 0.01 ） significantly in liver in PEO - M group, the effects were almost equal to SAA group （ P 〉 0.05 ）. Therefore, moderate intake of PEO exhibitted signifi- cantly protective effect on acute liver injury in mice.

关 键 词：牡丹籽油 小鼠 CCL4 急性肝损伤 保护作用 

分 类 号：R285.5[医药卫生—中药学]