双波长分光光度法测定烟叶中直链淀粉和支链淀粉含量的研究  

Determination of Contents of Amylose and Amylopectin in Tobacco Leaves by Dual Wavelength Spectrophotometry

作　　者：焦铭[1] 孙雪[1] 李笔[1] 黄俊潮[1] 

机构地区：[1]湖北科技学院基础医学院,湖北咸宁437100

出　　处：《安徽农业科学》2013年第24期9891-9892,共2页Journal of Anhui Agricultural Sciences

基　　金：湖北省教育厅科学研究计划项目(Q20122802;B2013050);湖北科技学院国家级大学生创新创业训练计划(201210927025)

摘　　要：[目的]检测初摘烟叶中直链淀粉和支链淀粉的含量。[方法]采用双波长分光光度法,选择直链淀粉检测波长为462和610 nm,支链淀粉检测波长为516和728 nm。根据标准样品的回归方程,可得到直链淀粉和支链淀粉的含量。[结果]直链淀粉的最大吸收波长为610 nm,支链淀粉的最大吸收波长为516 nm,在烟叶中含量分别为6.34%和20.41%。[结论]双波长分光光度法检测直链淀粉和支链淀粉操作简单,结果迅速,可用作批量检测。[ Objective ] To determine the contents of amylose and amylopectin in tobacco leaves. [ Method ] Dual wavelength spectrophotometry was used, and the measurement wavelengths of 462 and 610 nm for amylose, while 516 and 728 nm for amylopectin were selected accord- ing to the principle of dual wavelength calorimetric. The contents of amylose and amylopectin in tobacco＂ leaves were calculated based on re- gression ＇equation. [ Result] The maximum absorption wavelength were 610 and 516 nm respectively for the two complexes, and the contents were 6.34% and 20.41%. [ Conclusion] The contents of amylose and amylopectin in tobacco leaves determined with dual-wavelength spec- trophotometry were accurate, and the method was suitable for the determination of amylose and amylopectin in tobacco leaves samples.

关 键 词：双波长分光光度法 直链淀粉 支链淀粉 烟叶 

分 类 号：S572[农业科学—烟草工业]