产褐藻胶裂解酶菌株的分离与鉴定  

Isolation and identification of alginate lyase-producing bacteria

作　　者：黄李淑馨[1,2] 刘钢 岳敏[1,2] 曹海龙[1] 谭海东[1] 赵小明[1] 尹恒[1] 杜昱光[1] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116023 [2]中国科学院大学,北京100049 [3]大连海洋大学食品科学与工程学院,辽宁大连116023

出　　处：《食品工业科技》2013年第23期147-151,共5页Science and Technology of Food Industry

基　　金：国家高技术研究发展计划(863计划)项目(2011AA090704和2012AA021501);公益性行业(农业)科研专项(200903052);辽宁省博士启动基金(20111150);国家科技支撑计划(2013BAB01B01)

摘　　要：以褐藻酸钠为唯一碳源，从海南省红树林、天涯海角、黎安镇及海口等地采集的样品中分离获得4株高产褐藻胶裂解酶的菌株。通过3，5-二硝基水杨酸法监测该4株菌的发酵液酶活随培养时间的变化，结果表明当达到产酶高峰时，菌株E03的发酵液酶活最高（0．58U／mL），菌株$20和W14的发酵液酶活相当（0．23～0．27U／mL），W13的发酵液酶活稍低（0．13U／mL），但产酶规律呈现明显差异。由菌落形态特征结合16SrDNA系统发育分析，初步确定菌株E03属于假单孢菌属，菌株W14和W13属于弧菌属，而菌株$20属于黄杆菌属。Using sodium alginate as the sole carbon source,four bacteria with high productivity of alginate lyase were obtained from samples collected in mangrove, Tianya Haijiao, Li＇an Town and seaweed factory of Hainan Province,China.The alginate lyase activity in the medium was measured according to the method of dinitrosalicylic acid when the four bacteria were cultivated in the alginate medium.As a result,when the maximum enzyme activity was reached, alginate lyase activity in the medium of strain S20 was similar to that of strain W14 （0.23-0.27U/mL）, lower than that of strain E03 （0.58U/mL）, and higher than that of strain W13 （0.13U/mL）.However, the enzyme production rules of four bacteria showed significant differences. Based on the morphological properties and phytogenetic analysis of 16S rDNA,the strain E03 clustered with the genus Pseudornonas,the strains W13 and W14 grouped within the genus Vibrio,and the strain S20 classified as the genus Flavobacterium.

关 键 词：褐藻胶裂解酶 鉴定 黄杆菌 弧菌 假单胞菌 

分 类 号：TS201.3[轻工技术与工程—食品科学]