光合氧化酶联合化学氧化用于木纤维脱木质素  

Photosynthesis oxidases combined chemical oxidation used for delignification in lignocellulose

作　　者：熊燕飞[1] 陈筠[2] 甘玮[1] 王洋[1] 李培妹[1] 

机构地区：[1]武汉理工大学理学院生物技术系,湖北武汉430070 [2]武汉理工大学校医院化验室,湖北武汉430070

出　　处：《食品工业科技》2013年第23期168-171,共4页Science and Technology of Food Industry

摘　　要：光合氧化酶用于木质素降解,因受固态木质素与酶接触限制降解效率较低。TiO2、H2O2/Fe2+处理构树木质纤维后,再以光合氧化酶或漆酶进行酶促降解,明显提高了木质素的降解效率。结果显示,以纳米TiO2或H2O2/Fe2+联合处理构树木质纤维1.5h后再以光合氧化酶进行木质素降解1.5h,木质素的降解效率较单独以光合氧化酶降解3h分别提高36.0%和28.8%,以纳米TiO2或H2O2/Fe2+联合处理构树木质纤维1.5h后,再以漆酶降解1.5h,木质素的降解效率较单独以漆酶降解3h分别提高92.4%和85.0%。纳米TiO2和H2O2/Fe2+与光合氧化酶或漆酶联合作用,可有效提高木质素的酶促降解效率。The degradation of lignin by enzyme had a low degradation efficiency because it suffered restriction by the contact between solid state lignin and enzyme,which could be improved by combining the lightly toxic and tractable oxidant such as TiO2 and H2O2/Fe^2＋ and oxidase into the process of degradation. Brousso xylem pretreatmented by TiO2 or H2O2/Fe^2＋ then were degraded by photosynthesis oxidases or laccase in this paper.The results showed that papyrifera xylem fibre pretreatmented by nanometer-sized TiO2 or H2O2/Fe^2＋ for t.Sh and then by photosynthesis oxidases for 1.Sh, the degradation efficiency of lignin increased 36.0% or 28.8% respectively compared to those were degraded by photosynthesis oxidases for 3h alone.Conducted by nanometer-sized TiO2 for 1.Sh and then degraded by laccase for l.Sh,the degradation efficiency of lignin increased 92.4% or 85.0% compared to those were degraded by laccase for 3h.Consequently the degradation efficiency of lignin by photosynthesis oxidases or laccase could be facilitated efficiently by joint action with nanometer-sized TiO2 or H2O2/Fe^2＋.

关 键 词：光合氧化酶 漆酶 纳米TiO2 H2O2 FE^2+ 木质素降解 

分 类 号：TS201.2[轻工技术与工程—食品科学]