基于响应面分析法优化的蕨茎叶黄酮提取条件  

Optimization of extraction conditions of soluble flavonoids from Pteridium aquilinum var.latiusculum based on RSM

作　　者：林敏[1] 吴冬青[1] 任雪峰[1] 安红钢[1] 

机构地区：[1]甘肃省高校河西走廊特色资源利用省级重点实验室,河西学院,甘肃张掖734000

出　　处：《食品工业科技》2013年第23期211-215,共5页Science and Technology of Food Industry

基　　金：甘肃省教育厅研究生导师资助项目(1009-09);甘肃省高校河西走廊特色资源利用省级重点实验室科研资助项目(XZ1203)

摘　　要：目的:利用响应面法优化蕨老茎叶总黄酮的提取工艺条件。方法:以提取溶剂乙醇体积分数、液固比、提取温度及提取时间为影响因子,以提取总黄酮得率作为响应值,应用Box-Behnken中心组合设计建立数学模型,进行响应面法分析(RSM)。结果:蕨老茎叶总黄酮提取的最佳工艺为:乙醇体积分数53%,液固比68∶1,提取温度81℃,提取时间48min,理论得率13.83%,实际测得值为13.81%,两者较接近。结论:Box-Behnken设计结合响应面分析法可以很好地对蕨老茎叶总黄酮提取工艺进行优化。Objective： Response surface analysis methodology（RSM） was used to optimize the extraction technology of total flavonoids from Pteridium aquilinum var. latiusculum. Methods： Ethanol concentration, solvent-solid ratio, extraction temperature,extraction time were selected as influencing factors and yield of flavonoids as a response value,the experiment mathematical model was arranged according to Box-Behnken central composite experiment design and response surface analysis methodology （RSM）was implemented. Results： The optimal extraction conditions were as following： ethanol concentration 53 %, solvent-solid ratio 68：1, extraction temperature 81 ℃, and extraction time 48min, the predicted value of extraction rate of total flavonoids was 13.83% under the optimum conditions,and the experimental value was 13.81%.Conclusion, The combination of Box-Behnken design and response surface analysis could well optimize the extraction technology of total flavonoids from Pteridium aquilinum var. latiusculum.

关 键 词：蕨 黄酮 提取工艺 响应面分析 

分 类 号：TS201.1[轻工技术与工程—食品科学]