2,4,6-三氯苯酚降解菌的筛选及降解特性  

Study on 2,4,6-Trichlorophenol-degrading Bacterial Strain:Isolation,Identification and Biodegradation Characteristics

作　　者：范燕燕[1] 董昆明[1,2] 缪莉[1] 栾海彬 吉慧敏[1] 忻夏莹[1] 封克[1,2] 

机构地区：[1]扬州大学环境科学与工程学院,江苏扬州225127 [2]扬州大学海洋科学与技术研究所,江苏扬州225127

出　　处：《环境科学与技术》2013年第11期36-39,83,共5页Environmental Science & Technology

基　　金：国家自然科学基金项目(41006097);扬州大学科技创新培育基金(2012CXJ042);中国海洋大学海洋药物教育部重点实验室开放基金(KLMD(OUC)201203);江苏省高校自然科学基金(10KJB170013)

摘　　要：为了研究氯酚类环境激素2,4,6-三氯苯酚的生物降解性,从活性污泥中分离筛选出一株能以2,4,6-三氯苯酚为唯一碳源生长的降解性菌株T10,通过16S rRNA测序鉴定其为一种芽孢杆菌（Bacillus sp.）。在实验室条件下探讨pH值、温度等环境因素对菌株T10降解性的影响,实验结果表明该菌株在pH为7.0,30~40℃范围内具有高降解活性,底物浓度为25 mg/L时,120 h的降解率均能达到90%以上,在30℃条件下的降解半衰期为1.26 d。初始底物浓度达到30 mg/L时,2,4,6-TCP的抑制作用开始起主导作用,影响菌株降解活性。Biodegradation of 2,4,6-Trichlorophenol（2,4,6-TCP）,a kind of environmental hormones,was explored using a special bacterial strain designated as T10 which can use 2,4,6-TCP as the sole carbon source. Processes of isolation and identification were detailed and the degradation condition was optimized. The laboratory tests showed that under the condition of temperature of 30~40 ℃,pH value of 7.0 and initial 2,4,6-TCP concentration of 25 mg/L, the degradation rate,after culture of 120 h,could be as high as more than 90% and the biodegradation fit the first-order kinetic model with the rate constant 0.567. In addition,if the initial substrate concentration was increased to 30 mg/L,the biodegradation ability of T10would be subjected to repression.

关 键 词：2 4 6-三氯苯酚 生物降解 16S RRNA 

分 类 号：X172[环境科学与工程—环境科学]