菌株漂浮性对其柴油降解特性的影响  

Cellular Floating Activity of Diesel-oil-degrading Bacteria:Its Effects on Diesel-oil Degradation

作　　者：罗群[1,2] 张建国[2] 侯登勇[2] 何颖[2] 王庆蓉[2] 刘琼[2] 沈先荣[2] 

机构地区：[1]海军工程大学核能科学与工程系,湖北武汉430033 [2]海军医学研究所,上海200433

出　　处：《环境科学与技术》2013年第11期79-83,共5页Environmental Science & Technology

摘　　要：测定柴油降解菌的漂浮性、表面疏水性,比较不同漂浮性的菌株在不同搅动强度的海水中的柴油降解率。利用不同漂浮性的菌株构建降解菌群,比较其在低搅动海水中的柴油降解率。通过室内模拟实验,考察其实际应用效果。实验结果表明,菌株的漂浮性与其表面疏水性存在正相关性,具有漂浮性的菌株(Y8、C7、Y9)在低搅动海水中的降解率比高搅动情况下分别降低了22%、19%和17%,而不具有漂浮性菌株F6的降解率降低了53%。由漂浮性高的菌株(Y9、X1、S2)构建的降解菌群在低搅动海水环境中的柴油降解率明显高于其余菌群,10 d后,达到了57%。室内模拟实验表明,在添加漂浮性菌群和亲油肥料S200、水溶性营养盐的实验组中,柴油降解率在7 d后分别达到了87%和84%。Effective strains of diesel-oil degrading bacteria were isolated from seawater contaminated by oil and their cellular floating activity and cell surface hydrophobicity were detected. To build the most effective bacterial colony for assimilating diesel oil,experiments were conducted to compare different groups of bacterial strains regarding their floating activities at different strength of agitation. The findings of the study suggested that cellular floating activity of each strain was positively correlated with cell surface hydrophobicity;strains without floating activity such as F6 showed stronger diesel-oil degrading capacity in intensified agitation（180 r/min）,much higher than that in declined agitation（80 r/min）. In general,strains having higher cellular floating activity displayed remarkable diesel-oil degrading capacity in slow agitation. The effective bacterial colony made up of strains with higher cellular floating activity including Y9,X1 and S2 degraded diesel oil by 57% within the period of 10 days. Furthermore,by addition of a kind of oloephilic fertilizer S200 and soluble nutrients,bacterial colony with floating activity could greatly upgrade its diesel-oil biodegrading capacity.

关 键 词：海水 柴油 降解 漂浮性 表面疏水性 

分 类 号：X172[环境科学与工程—环境科学]