柯萨奇B3病毒5'非编码区基因序列及遗传进化分析  

Analysis on gene sequence and genetic evolution of 5'untranslated region of Coxsack-ievirus B3

作　　者：李哲[1] 周密[2] 李凡[1] 

机构地区：[1]吉林大学白求恩医学院,吉林长春130021 [2]长春医学高等专科学校

出　　处：《中国妇幼保健》2013年第34期5706-5708,共3页Maternal and Child Health Care of China

基　　金：吉林省高校新世纪科学技术优秀人才支持计划〔2013540〕;吉林省教育厅"十一五"科学技术研究项目〔2009551〕

摘　　要：目的:研究柯萨奇B3病毒(CVB3)/MKP株5'非编码区基因序列及变异性,探讨其致病性的分子基础。方法:用Hela细胞培养柯萨奇B3病毒,RT-PCR法扩增5'非编码区,测序后进行同源性、变异性及遗传进化分析。结果:CVB3/MKP株5'非编码区基因全长742个核苷酸,该株病毒与CVB3/20、CVB3/28、CVB3/0、CVB3/Nancy、CVB3/AS、CVB3/Woodruff(CVB3/W)及CVB3(CXA3CG)株5'非编码区同源性在91.9%～99.6%之间,而与CVB3/GA和CVB3/CO两种病毒株同源性分别为83.6%和83.3%。CVB3/MKP株的5'非编码区序列与既致胰腺炎又致心肌炎的病毒株共同聚簇,而与非致病毒株和仅致胰腺炎的病毒株距离较远。结论:CVB3/MKP株5'非编码区基因序列可能与病毒的毒力表型有关。Objective： To study the gene sequence and vari- ation of 5 ＂untranslated region of Coxsackievirus B3 （ CVB3 ） /MKPstrain, explore the pathogenic molecular foundation. Methods： CVB3 was cultured in HeLa cells, RT - PCR was used to amplify 5＂untranslated region of CVB3, and then homology analysis, variation analysis, and genetic evolution analysis were carried out after gene sequencing. Results： There were 742 nucleotides in 5＂untranslated region of CVB3/MKP strain, the homologies of CVB3/MKP strain and CVB3/20, CVB3/28, CVB3/0, CVB3/Nancy, CVB3/AS, CVB3/ Woodruff （CVB3/W）, CVB3 （CXA3CG） strains were 91.9% -99.6%, but its homologies with CVB3/GA and CVB3/CO were 83.6v/r and 83.3% , respectively. The 5＂untranslated region of CVB3/MKP strain clustered together with virus strains indncing panereatitis and luyoearditis, hut it was apart from non - virulence strains and strains inducing pancreati- tis. Conclusion： The gene sequence of 5＂untranslated region of CVB3/MKP strain is correlated with viral virulence phenotype.

关 键 词：柯萨奇B3病毒 基因序列 系统发生树 

分 类 号：R725.4[医药卫生—儿科]