小剂量辣椒素对大鼠胃缺血再灌注胃黏膜的保护作用  

The protective effect of minidose capsaicine on gastric mucosa injury of gastric ischemia-reperfusion in rats

作　　者：马虹飞 宋蕾[2] 邵长卿[1] 冯国营[3] 

机构地区：[1]山东省青岛市肿瘤医院外二科,266042 [2]山东省青岛市肿瘤医院放疗一科,266042 [3]滨州医学院人体解剖与组织胚胎学研究所

出　　处：《中国临床研究》2013年第11期1144-1145,1148,I0001,共4页Chinese Journal of Clinical Research

摘　　要：目的研究小剂量辣椒素(capsaicine,Cap)对大鼠胃缺血再灌注(gastric ischemia-reperfusion,GI-R)后胃黏膜损伤的保护作用。方法 SD大鼠18只随机分为假手术(Sham)组、GI-R组(又分为Cap组和Cap对照组)3组,每组6只。Cap组和Cap对照组于GI-R模型建立24 h后、Sham组于假手术24 h后,取大鼠胃组织。按Guth标准计数胃黏膜损伤指数;HE染色行组织病理学观察。结果 GI-R后胃黏膜弥漫性充血水肿及点片状出血糜烂,Cap组较Cap对照组表现轻。Sham组、Cap组和Cap对照组胃损伤指数分别为:3.20±1.81、31.12±12.13和60.25±23.76,3组比较差异有统计学意义(P<0.01,P<0.05)。HE染色组织病理学检查,Cap组较Cap对照组病理损伤明显减轻。结论小剂量Cap能降低GI-R大鼠的胃黏膜损伤指数,对胃有明显的保护作用。Objective To investigate the protective effect of minidose capsaicine（Gap） on gastric mucosa injury after gastric ischemia-reperfusion in rats. Methods Sprague-Dawley rats were randomly divided into sham- operated （Sham） group and gastric ischemia-reperfusion （GI-R） group, and the latter was randomly divided into two groups： Cap group and Cap control group （n =6,each group）. Rats were sacrificed 24 h after GI-R model established or sham-operated, and gastric tissue was obtained. Gastric mucosal injury index was counted and standard by Guth under stereomicroscope. Histopathological changes in the gastric mucosa were investigated by HE staining under lightmicroscope. Results After GI-R, diffuse mucosal edema and bleeding point sheet erosion was found un- der stereomicroscope. Compare to Cap control group, Cap group exhibited mild injury. Guth scores of Sham group, Cap group and Cap control group were 3.20 ±1.81,31.12 ± 12.13 and 60.25 ±23.76, respectively. Score difference among the three groups was statistically significant （ P 〈 0.01, P 〈 0. 05 ）. The pathological damage of Cap group was milder than Cap control group by HE staining. Conclusion Application of minidose Cap can reduce the gastric mucosal injury index in GI-R.

关 键 词：辣椒素 胃黏膜损伤 胃缺血再灌注 大鼠 

分 类 号：R573[医药卫生—消化系统]