基质金属蛋白酶-10基因多态性与颈动脉斑块易损性的相关性  

Association between matrix metalloproteinase-lO gene polymorphisms and instability of carotid plaque

作　　者：朱峰[1] 金笑平[1] 黄米武[1] 朱敏[2] 陈巧玲[1] 王凤[1] 胡晓飞[1] 王皖芬[1] 李卫玲[1] 李彩[1] 朱宗亚[1] 郑周[1] 

机构地区：[1]温州医学院附属台州医院神经内科,浙江省台州市317000 [2]温州医学院附属台州医院神经内科中心实验室,浙江省台州市317000

出　　处：《中华医学遗传学杂志》2013年第6期711-715,共5页Chinese Journal of Medical Genetics

基　　金：浙江省自然科学基金（LY13H290003）;浙江省中医药科学研究基金计划及攀登工程建设项目（2011ZA111）;台州市科技资金项目（111KY0603）

摘　　要：目的探讨基质金属蛋白酶-10（matrixmetalloproteinase-10，MMP=10）基因两个外显子区单核苷酸多态性与颈动脉斑块易损性的关系。方法585例符合纳入标准无排除标准的住院患者根据颈动脉超声分为易损斑块组（共206例）和非易损斑块组（共379例）。采用实时荧光定量PCRTaqMan法进行基因分型。结果rsl7435959位点的Gc＋CC基因型频率和c等位基因频率在易损斑块组和非易损斑块组之间的差异均有统计学意义（P=0．006，OR=2．012；P=0．001，OR=2．160）；经Logistic回归校正后仍有统计学意义（P=0．007，OR=2．022；P=0．002，OR=2．104）。rsl7293607位点T等位基因的频率为0．56％。结论MMP=10基因rsl7435959多态性可能与中国汉族人群颈动脉易损斑块的发生有关；rsl7293607对本研究基于的人群而言并非真正的多态性。Objective To assess the association between 2 single nucleotide polymorphisms （SNPs） located in exonic regions of matrix metalloproteinase-10 （MMP-10）gene and instability of carotid plaques in a Han Chinese population. Method Five hundred and eighty-five patients were divided into carotid vulnerable plaque group （n =206） and stable plaque group （n =379） based on results of carotid D-mode ultrasonography. The SNPs were genotyped by real-time polymerase chain reaction using an ABI 7300 TaqMan platform. Results The distribution of rs17435959 between the two groups was significantly different at both genotypic （GC＋CC vs. GG, P=0. 006, OR=2. 012） and allelic levels （C vs. G, P= 0. 001,OR= 2. 160）. Above differences have remained significant with binary logistic regression analysis （P=0.007, 0R=2.022; P=0.002, 0R=2.104）. The minor allele frequency of rs17293607 was0.56%. Conclusion Above findings suggested that rs17435959 of the MMP-IO gene is associated with carotid vulnerable plaque in ethnic Chinese Hans. The C allele may be a susceptible predictor for carotid vulnerable plaque.

关 键 词：基质金属蛋白酶-10 多态性 疾病遗传易感性 颈动脉疾病 缺血性脑卒中 

分 类 号：R743[医药卫生—神经病学与精神病学]