牛磺酸抑制亚硒酸钠性白内障的FT拉曼及FT红外光谱分析  

FTRaman and FTIR spectroscopy of selenite cataractous lenses protected by taurine

作　　者：张伟[1] 陈翠真[1] 张煦[2] 许振华[2] 

机构地区：[1]北京市眼科研究所,100730 [2]北京大学稀土材料化学及应用国家重点实验室

出　　处：《眼科研究》2000年第6期506-508,共3页Chinese Ophthalmic Research

基　　金：北京大学稀土材料化学及应用国家重点实验室基金资助

摘　　要：目的　探讨亚硒酸钠性白内障中晶状体蛋白成分和结构的变化以及牛磺酸对这些变化的抑制作用。方法　首次用傅里叶变换拉曼光谱和红外光谱同步研究牛磺酸抑制亚硒酸钠性白内障晶状体中一些基因伸缩振动的变化。结果　亚硒酸钠性白内障晶状体中巯基和色氨酸的伸缩振动明显降低 ,蛋白质分子一些基团的伸缩振动在白内障形成之初就发生明显改变 ,牛磺酸可抑制上述变化。结论　亚硒酸钠性白内障形成之初 ,晶状体蛋白中SH (巯基 )、trp (色氨酸 )、phe (苯丙氨酸 )和CH2 (亚甲基 )等就受到损伤 ,牛磺酸可保护这些基团 。ObjectiveTo investigate the changes of composition and structure of lens protein in selenite cataract as well as the preventive effect of taurine on these changes.MethodsFourier Transform Raman and infrared spectroscopy(FTRaman and FTIR)were used to investigate the changes of some important groups both in selenite cataractous lenses and in the lenses protected with taurine.ResultsThe peaks of SH group and trypsine from selenite cataractous lenses decreased sharply.The changes of some protein groups changed obviously in the early stage of cataractogenesis.Taurine could attenuate these changes.ConclusionIn the early stage of selenite cataract,the SH group,tryp ,phe and CH 2 group in crystalline are damaged.Taurine can protect these groups.It might be the mechanism of the prevention of selenite cataract with taurine.

关 键 词：光谱分析 白内障 牛磺酸 

分 类 号：R776[医药卫生—眼科]