灰树花胞外多糖提取工艺优化  

Optimization of Extraction for Exopolysaccharides from Grifola Frondosa

作　　者：汤庆莉[1] 张洪[2] 张勇[2] 王娜[2] 付红伟[3] 吴天祥[2] 

机构地区：[1]贵州大学化学与化工学院,贵州贵阳550025 [2]贵州大学生命科学学院,贵州贵阳550025 [3]浙江大学生物医学工程与仪器科学学院,浙江杭州310027

出　　处：《核农学报》2013年第11期1704-1708,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31060272)

摘　　要：通过对乙醇浓度、醇沉时间、醇沉温度3个单因素试验对灰树花胞外多糖提取过程中的变化规律进行研究,并且通过正交试验法,以灰树花EPS提取量为考察指标,对灰树花胞外多糖提取工艺条件进行优化。结果表明:灰树花胞外多糖的最佳提取条件为乙醇浓度95%、醇沉时间10h、醇沉温度4℃。在此条件下灰树花胞外多糖的提取量为321.94mg·L-1,较未优化的灰树花胞外多糖提取条件提高了57.79%。In this work, the effects of ethanol volume percentage, extraction time and extraction temperature on the change of EPS content during the extraction of exopolysaecharides from Grifola frondosa were explored through single- factor and orthogonal array experiments on the basis of EPS content. The optimal extraction conditions for EPS were optimized to be extracted at 4℃ following extraction for 10 h in ethanol with a volume percentage adjusted to 95% , in which the extraction content of EPS from Grifola frondosa reached to 321.94mg· L^-1, and the extraction rate increased by 57.79% comprised with the unoptimized group.

关 键 词：灰树花 胞外多糖(EPS) 提取条件 正交试验 

分 类 号：TQ914[化学工程]