响应面法优化超声辅助提取金银木果实色素工艺  

Optimizing Ultrasonic-Assisted Extraction of Pigment from Lonicera maackii Fruit by Response Surface Methodology

作　　者：王立志[1] 唐克华[1] 

机构地区：[1]吉首大学林产化工工程湖南省重点实验室,湖南张家界427000

出　　处：《食品科学》2013年第22期48-53,共6页Food Science

基　　金：湖南省高校科技创新团队支持计划项目(湘教通(2010)212号);湖南省研究生科技创新基地项目(201203A)

摘　　要：对金银木破碎果实首先用溶剂恒温浸提,然后进行超声萃取处理以提取色素。采用响应面优化金银木果实色素超声辅助提取工艺的料液比、超声时间和超声功率。结果表明:料液比、超声时间和超声功率对金银木果实色素超声辅助提取均有显著影响;最优工艺条件为料液比1:15(g/mL)、超声时间30min和超声功率500W;在最优工艺条件下超声提取的金银木果实色素样液稀释10倍后,在450nm波长处的吸光度理论预测值为0.836,而实际实验的吸光度为0.827,二者数值仅相差1.08%。因此,运用响应面法优化的超声辅助提取工艺条件可行。Following a period of spontaneous solvent extraction at a constant temperature, ultrasonication was applied to enhance pigment extraction from crushed Lonicera maacldi fruit. Three extraction parameters including material/solvent ra- tio, ultrasonication time and power were optimized by using response surface methodology. All the operating parameters had a significant effect on extraction efficiency and the optimal extraction conditions were determined as 1：15 （g/mL）, 30 rain and 500 W for material/solvent ratio, ultrasonication time and power, respectively. The predicted absorbance at 450 nm of the pigment extracted under the optimized conditions after 10-fold dilution was 0.836, showing a relative error of only 1.08% in comparison to the experimental absorbance of 0.827. Thus the optimized process established using response surface meth- odology is feasible for pigment extraction from Lonicera maacldi fruit.

关 键 词：金银木 色素 超声提取 响应面法 

分 类 号：TS202.3[轻工技术与工程—食品科学]