用于亚硝酸盐快速检测的三维纸质微流控芯片的制作  

Fabrication and Analytical Application of Three-Dimensional Paper-Based Microfluidics for Rapid Detection of Nitrite

作　　者：肖良品 刘显明[1,3] 刘启顺[1] 钟润涛[1,3] 周小棉[4] 林炳承[1,3] 杜昱光[1,3] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116023 [2]中国科学院大学,北京100049 [3]芯片实验室研究中心(中国大连),辽宁大连116023 [4]广州市第一人民医院,广东广州510180

出　　处：《食品科学》2013年第22期341-345,共5页Food Science

基　　金：国家重大科技专项(2013ZX09507005-006-001)

摘　　要：选用中速定性滤纸作为纸芯片制作材料，采用装订法制作三维纸芯片，以63．4g／L柠檬酸溶液、8．61g／L对氨基苯磺酰胺、2．59g／LN-（1．萘基）乙二胺盐酸盐作为显色剂，结合实验室自制的比色检测装置用于亚硝酸盐的检测。结果表明：设计制作的三维纸质微流控芯片结合自制的比色检测装置，可以实现亚硝酸盐的快速定量检测，在0～10mg／L的质量浓度范围内有良好的线性，序为0．9920，最低检测限为2mg／L，加标回收率为91．4％～102．0％，制作的亚硝酸盐检测纸芯片比较稳定，在室温条件下放置7周，得到的显色结果与新制作的纸芯片的显色结果基本一致。亚硝酸盐检测纸芯片简单易用、成本低、耗样量少、灵敏度较好，能快速实现亚硝酸盐的检测。Medium-speed qualitative filter paper was employed to fabricate paper-based microfluidics and 3D paper-based microfluidics were integrated by wax printing-bookbinding method; a nitrite indicator containing 63.4 g/L citric Acid, 8.61 g/L sul- fanilamide and 2.59 g/L N-（1-naphthyl） ethylenediamine coupled with a lab-prepared colorimetric detection device was used for nitrite determination. The 3D paper-based microfluidics could rapidly test nitrite, and the image acquired was analyzed using a gray model for gray intensity representing the concentration of nitrite. The linear range was 0-10 mg/L, with cor- relation coefficient of 0.9920. The detection limit of the method was 2 mg/L, and the recovery of nitrite from spiked samples was 91.4%-102.0%. After stored under normal conditions and 4 ℃ for 7 weeks, the color intensity of the paper-based micro- fluidics for nitrite detection were little change. The 3D paper-based microfluidics can provide a simple, easy to use, low-cost and sensitive approach for rapid detection of in foods with small sample consumption.

关 键 词：纸质微流控芯片 三维 亚硝酸盐 快速检测 

分 类 号：TS207.3[轻工技术与工程—食品科学]