核转录因子-κB/p65在口腔鳞癌患者中的表达研究  

Study on expression of NF-KB/p65 in oral squamous cell carcinoma

作　　者：李玉华[1] 王海欣[2] 张爱军[3] 

机构地区：[1]唐山市协和医院口腔科,063004 [2]唐山市中医医院口腔科 [3]唐山市第八医院口腔科

出　　处：《现代口腔医学杂志》2013年第6期349-351,共3页Journal of Modern Stomatology

基　　金：唐山市科技局课题(13130265a)

摘　　要：目的通过对NF-κB/p65在口腔鳞癌中表达进行研究,初步探讨它们在口腔鳞癌中的作用,为临床治疗提供一种新的思路。方法无菌提取口腔癌患者及其对照外周血单核细胞,利用实时定量PCR检测p65的基因表达,通过Western blot检测单核细胞中的p65的磷酸化水平,并通过免疫组化技术检测癌组织中的p65的表达水平的差异。结果口腔癌患者外周单核细胞p65的基因表达明显增加,p65磷酸化水平也明显增强。口腔癌组织中p65的磷酸化水平较正常组织明显明显增加。结论核转录因子-κB/p65在口腔癌的发生发展过程中被大量表达并大量被激活,可能在其侵袭和转移中发挥重要作用,以此将为口腔癌的发病机理及治疗提供分子生物学基础。Objective To investigate the expression of NF-κB/p65 oral squamous cell carcinoma.and to evaluate their functions in tumorigenesis and progession in oral squamous cell carcinoma.So as to provide a potential new way for theraputies of oral squamous cell carcinoma. Methods The gene of expressions of NF-κB/p65 were measured by RT- PCR of monocyte in oral squamous cell carcinoma.And the expression of NF-K B/p65 protein were detected by Western blot. Immunohistochemical staining technique was used to determine the expression NF-κB/p65 in all specimens. Results NF-κB/p65 gene in monocyte of oral squamous cell carcinoma was up-regulated detected by realtime PCR;Western Blot showed that phosphor-p65 were high expressed in monocyte of oral squamous cell carcinoma.Moreover, Immunohistochemical staining further show that the activation of p65 protein in oral squamous cell carcinoma. Conclusion The result suggests that oral squamous cell carcinoma have higher NF-κB activity which may significantly contribute to tumor invasion and metastasis.NF-ttB and its downstream or upstream signaling effectors may be used as effective molecular therapy targets of oral squamous cell carcinoma.

关 键 词：口腔鳞癌 NF-ΚB P65 

分 类 号：R739.8[医药卫生—肿瘤]