茶多酚诱导鼻咽癌细胞caspase-3活化  

Tea polyphenols induce the activity of caspase-3 in nasopharyngeal carcinoma cells

作　　者：罗非君[1] 胡智[1] 邓锡云[1] 顾焕华[1] 曹亚[1] 

机构地区：[1]湖南医科大学肿瘤研究所,湖南长沙410078

出　　处：《癌症》2000年第12期1082-1086,共5页Chinese Journal of Cancer

基　　金：国家自然科学杰出青年基金!(39525022);国家科技部恶性肿瘤发生与发展的基础性研究基金!(G1998051201)资助

摘　　要：目的：探讨茶多酚能否诱导鼻咽癌细胞系 CNE1-LMP1凋亡及凋亡发生的分子机制。方法：用不同浓度的茶多酚处理 CNE1-LMP1细胞，分别用荧光显微镜、 DNA琼脂糖凝胶电泳、 MTT和流式细胞仪等方法，观察细胞生化和形态学改变；同时，确定茶多酚诱导细胞凋亡过程中 caspase-3活性的动力学变化。结果：茶多酚处理后，荧光显微镜下可见 CNE1-LMP1细胞在形态学上出现细胞核固缩、核碎裂、凋亡小体等凋亡细胞的形态学改变；琼脂糖凝胶电泳 DNA呈典型的“梯状”条带； MTT测定发现茶多酚处理后细胞生长受到不同程度的抑制。同时， CNE1-LMP1细胞在凋亡过程中 caspase-3活性的升高，呈明显的时效关系。结论：茶多酚能诱导 CNE1-LMP1细胞凋亡，可能通过 caspase-3的活化诱导细胞凋亡。Objective: The current study was designed to investigate the apoptosis induced by tea polyphenols in nasopharyngeal carcinoma cell line CNE1-LMP1 and molecular mechanism in the apoptotic process. Methods: After treatment of CNE1-LMP1 cells with various concentrations of tea polyphenols,apoptosis of the cells was detected by fluorescent microscopy, DNA gel electrophoresis, MTT assay and flow cytometry, and the activity of caspase-3 was detected by Western blot analysis and caspase-3 assay at different time points. Results:Typical nuclear condensation was observed under fluorescent microscopy, DNA Ladder appeared on the 2％ agarose gel. Survival rate of the cells was decreased. Furthermore, the activity of caspase-3 induced by tea polyphenols in time-dependent manner during apoptotic process. Conclusion: Tea polyphenols could induce apoptosis of CNE1-LMP1 and the activity of caspase-3 might be involved in the apoptotic process.

关 键 词：鼻咽肿瘤 CASPASE-3 细胞凋亡 茶多酚 

分 类 号：R739.63[医药卫生—肿瘤]