褐藻降解菌群筛选及其复合酶固定化研究  

Screening of algae degradation strains and their immobilized compound enzyme

作　　者：杜冰[1,2] 郑来久[1,2] 

机构地区：[1]江南大学纺织服装学院,无锡214122 [2]大连工业大学辽宁省清洁化纺织重点实验室,大连116034

出　　处：《工业微生物》2013年第6期39-42,共4页Industrial Microbiology

基　　金：辽宁省博士启动基金项目(20111069);辽宁省创新团队项目(LT2010010)

摘　　要：以大连地区的褐藻为材料，筛选了褐藻降解菌群，其在30℃、pH7．5条件下培养74h时，酶活达1．883IU／mL。利用硅藻土吸附法经冷冻干燥制备了固定化复合酶，分析了复合酶系的酶学性质，其最适反应温度为45℃，并在40℃～55℃范围内具有良好的热稳定性；最适pH为7．5，并在pH7．0～8．5之间pH稳定性良好。利用固定化酶进行褐藻酸钠制备，提取率达48．3％，粘度为2．9Pa·s，与传统方法相比均有显著提高，为工程化生产褐藻酸钠提供一定基础。The algae degradation strains were screened based on the brown algae from Dalian area. After being cultured for 74 h at 30 ℃ and pH 7.5, the compound enzyme activity was 1. 883 IU. The immobilized compound enzyme was made by freeze dry preparation and the properties of the immobilized enzyme were analyzed as follows ： the optimum temperature was 45 ℃, and the thermal stability was better between 40 ℃ ~ 55 ℃ ; the optimum pH was 7.5, and the pH stability was better between 7.0 - 8.5. The immobilized compound enzyme was then used to extract alginate. The results indicated that the extraction rate was 48.3% and the viscosity was 2.9 Pa .s. Compared with traditional methods, the data was sig- nificantly better than before and provided the basis for engineering production.

关 键 词：褐藻 菌群筛选 复合酶 固定化 褐藻酸钠 

分 类 号：TQ925[轻工技术与工程—发酵工程]