巨大芽孢杆菌B.m 2980产孢对氧化葡萄糖酸杆菌产酸的影响  

Effects of Bacillus megaterium 2980's spore formation on 2 KGA production of Gluconobacter oxydans

作　　者：宫晓丽[1] 郭智勇[1] 吕淑霞[1] 于晓丹[1] 马镝[1] 张良[1] 张忠泽[2] 陈宏权 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110866 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016 [3]东北制药总厂Vc公司,辽宁沈阳110028

出　　处：《工业微生物》2013年第6期49-53,共5页Industrial Microbiology

基　　金：维生素C混合菌发酵中伴生菌的AP36活性蛋白基因克隆表达及其对产酸菌作用的分子机制研究;沈阳市科技计划项目(编号F10-205-1-55);Vc二步发酵混合菌中伴生菌对产酸菌的作用机制研究;沈阳农业大学NSFC启动基金资助项目(编号:20102002)

摘　　要：为确定维生素C二步发酵中巨大芽孢杆茵(伴生菌)芽孢形成对氧化葡萄酸杆菌(产酸菌)产酸的影响,本研究通过对巨大芽孢杆菌生长特性分析,选取培养12 h(未形成芽孢)和36 h(芽孢大量形成)巨大芽孢杆茵B.m 2980,检测其胞外液、胞内液以及混合液对产酸茵生成2-酮基-L-古龙酸的影响。结果表明,在未开始形成芽孢时,伴生菌胞外液、胞内液及混合液对产酸菌的生长和产酸有较低的促进作用,其中胞内液的促进能力大于胞外液;在芽孢生成后,胞外液以及混合液对产酸茵生长和产酸的促进能力显著提高。In order to determine the relationship between the spore formation of Bacillus megaterium 2980 （ B. m 2980, called companion bacterium） and Gluconobacter oxydans （ G. o, called acid-producing strain） in vitamin C two-step fermen- tation, the effects of the culture supernatant, the cytosol and the mixed component solution of companion bacterium which cultured for 12 h （not forming spores） and 36 h （forming spores） on the growth of G. o and its synthesizing ability of 2- keto-L-gulonic acid （2-KGA） was studied through the analysis of B. m 2980 growth characteristics. The results showed that when the B. m 2980 spores did not form, the culture supernatant, the cytosol and mixed component solution had low effects on the growth of acid-producing strain and its 2-KGA production. The cytosol of B. m 2980 promoted the growth and 2 KGA production of G. o greater than that of the culture supernatant. After the spore formation of B. m 2980, the culture supernatant and mixed component solution of B. m 2980 affected growth and 2-KGA production of G. o significantly.

关 键 词：维生素C二步发酵 2-酮基-L-古龙酸 胞外液 胞内液 混合液 

分 类 号：Q933[生物学—微生物学]