在线扫集-胶束毛细管电动色谱法分离测定急支糖浆中阿魏酸、原儿茶醛和原儿茶酸  

Determination of Fumalic Acid,Protocatechuic Aldehyde and Protocatechuic Acid in Jizhitangjiang by Sweeping-Micellar Electrokinetic Chromatographic Method

作　　者：李利军[1] 张瑞瑞[1] 孙科[1] 杨兰兰[1] 崔福海[1] 罗应[1] 李彦青[1] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006

出　　处：《分析科学学报》2013年第6期777-780,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.20665001);广西自然科学基金(No.0832062)

摘　　要：本文采用扫集-胶束毛细管电动色谱法(Sweeping-MEKC)分离测定急支糖浆中的阿魏酸、原儿茶醛和原儿茶酸。采用未涂层熔融石英毛细管(50cm×50μm,有效柱长36cm),环境温度25℃,缓冲体系为20mmol/L NaH2PO4+80mmol/L十二烷基磺酸钠(SDS)+12.5%乙腈(V/V)(pH=2.2),紫外检测波长225nm,运行分离电压-20kV,进样时间60s,达到最佳的分离效果。在优化条件下,阿魏酸、原儿茶醛和原儿茶酸均在15min内出峰,峰面积的相对标准偏差(RSD)均小于5%,检出限分别为109.95μg/L、88.48μg/L和15.96μg/L。A sweeping-micellar electrokinetic chromatography （sweeping-MEKC） was used for the separation and determination of fumalic acid, protocatechuic aldehyde and protocatechuic acid in Jizhitangjiang. The result indicated that a best separation of the three compounds was obtained on a uncoated fused silica capillary column（50 cmX 50 μm,effective length 36 cm） by using the background electrolyte containing 20 mmol/L NaH~ PO4,80 mmol/L SDS and 12.5 ~//0 （by volume） acetonitrile at pH 2.2 with ultraviolet detection wavelength of 225 nm,separation voltage of --20 kV and injection time of 60 s at 25 ~C. Under the optimum conditions, the separation of the three compounds was performed within 15 minutes with relative standard deviations（RSDs） of peak area less than 5~. The detection limits（S/N= 3） of fumalic acid, protocatechuic aldehyde and protoeatechuic acid were 109. 95 μg/L, 88.48 μg/L and 15.96 ptg/L,respectively.

关 键 词：扫集一胶束电动毛细管色谱 阿魏酸 原儿茶醛 原儿茶酸 

分 类 号：O657.8[理学—分析化学]