碳源和氮源对木蹄层孔菌产漆酶的影响及酶学性质研究  

The Effect of Carbon and Nitrogen Sources on Laccase Production and Properties from Fomes fomentarius

作　　者：尚洁[1,2] 吴秋霞[2] 练小龙 王秋玉[1] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040 [2]北方民族大学生物科学与工程学院,银川750021

出　　处：《中国生物工程杂志》2013年第11期32-37,共6页China Biotechnology

基　　金：黑龙江省科技攻关重大项目资助(GA06B301)

摘　　要：对不同碳源和氮源及碳氮比对木蹄层孔菌产漆酶的影响进行了研究,以提高漆酶产量,同时分析了该漆酶的酶学性质。漆酶合成的最适碳源是麦麸,最适氮源是蛋白胨,C∶N为10.4,优化后漆酶产量增加了约7.88倍;反应最适pH为3.0,最适反应温度为50℃,K m为0.20 mmol/L,V max为2.58 mmol/L·min;DTT 0.1mmol/L和NaN310mmol/L几乎能抑制漆酶全部活性,终浓度为10mmol/L的Ba2+,Ca2+,Co2+和Fe2+也几乎能抑制该酶的全部活性。该研究将为木蹄层孔菌漆酶的生产及在环境工程领域的应用提供基础信息。The production of laccase by Fomes fomentarius was studied. Cultures conditions involving variations in carbon and nitrogen sources and different C： N ratios were examined at constant temperature and pH, with the aim of increasing yield of laccase. And its enzymatic properties were determined. The best results were obtained when using wheat bran and peptone as carbon and nitrogen sources respectively with a C： N ratio of 10. 4. Compared to the initial medium, the highest laccase yield observed is approximately increased by 7.88 times under the optimized conditions. The optimum pH and temperature for its activity is 3.0 and 50C with the corresponding Km and Vmax of 0. 20 mmol/L and 2.58 mmol/L min respectively. DTI＇（0. lmmol/L） and NaN3 （10mmol/L） nearly inhibit all activity of the laccase, as well as the metal ions especially Ba2＋ , Ca2＋ , Co2＋ and Fe2 ＋ （10mmol/L）. In summary, our results will provide basic information to the production of laccase by Fomes fomentarius and the utilization of environmental engineering in the future.

关 键 词：木蹄层孔菌 漆酶碳源和氮源 酶学性质 

分 类 号：X172[环境科学与工程—环境科学]