Role of endoplasmic reticulum stress in the loss of retinal ganglion cells in diabetic retinopathy  

作　　者：Liping Yang Lemeng Wu Dongmei Wang Ying Li Hongliang Dou Mark O.M.Tso Zhizhong Ma 

机构地区：[1]Department of Ophthalmology,Peking University Third Hospital,Key Laboratory of Vision Loss and Restoration,Ministry of Education [2]Wilmer Eye Institute,Johns Hopkins University School of Medicine

出　　处：《Neural Regeneration Research》2013年第33期3148-3158,共11页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.81170877;the National Basic Research Program of China,No.2007CB512203

摘　　要：Endoplasmic reticulum stress is closely involved in the early stage of diabetic retinopathy. In the present study, a streptozotocin-induced diabetic animal model was given an intraperitoneal injection of tauroursodeoxycholic acid. Results from immunofluorescent co-localization experiments showed that both caspase-12 protein and c-Jun N-terminal kinase 1 phosphorylation levels significantly in- creased, which was associated with retinal ganglion cell death in diabetic retinas. The C/ERB ho- mologous protein pathway directly contributed to glial reactivity, and was subsequently responsible for neuronal loss and vascular abnormalities in diabetic retinopathy. Our experimental findings in- dicate that endoplasmic reticulum stress plays an important role in diabetes-induced retinal neu- ronal loss and vascular abnormalities, and that inhibiting the activation of the endoplasmic reticulum stress pathway provides effective protection against diabetic retinopathy.Endoplasmic reticulum stress is closely involved in the early stage of diabetic retinopathy. In the present study, a streptozotocin-induced diabetic animal model was given an intraperitoneal injection of tauroursodeoxycholic acid. Results from immunofluorescent co-localization experiments showed that both caspase-12 protein and c-Jun N-terminal kinase 1 phosphorylation levels significantly in- creased, which was associated with retinal ganglion cell death in diabetic retinas. The C/ERB ho- mologous protein pathway directly contributed to glial reactivity, and was subsequently responsible for neuronal loss and vascular abnormalities in diabetic retinopathy. Our experimental findings in- dicate that endoplasmic reticulum stress plays an important role in diabetes-induced retinal neu- ronal loss and vascular abnormalities, and that inhibiting the activation of the endoplasmic reticulum stress pathway provides effective protection against diabetic retinopathy.

关 键 词：neural regeneration peripheral nerve injury endoplasmic reticulum stress diabetic retinopathy injury of retinal ganglion cells M011er cells ASTROCYTES c-Jun N-terminal kinase caspase-12 protein C/ERB homologous protein retinal microcirculation glial fibrillary acidic protein grant-supportedpaper NEUROREGENERATION 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]