siRNA抑制stathmin表达对高级别胶质瘤血管内皮细胞迁移的影响  

Inhibition of Stathmin Expression by siRNA Influence on the Invasion of Vascular Endothelial Cell of High Grade Glioma

作　　者：乔万臣[1] 杨阿敏[2] 慕璐岩[1] 秦向英[1] 刘晓谦[1] 

机构地区：[1]哈尔滨医科大学附属第四临床医院,黑龙江哈尔滨150001 [2]北京医科大学附属第一医院,北京100034

出　　处：《现代生物医学进展》2013年第32期6268-6271,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨siRNA抑制Stathmin表达对高级别胶质瘤微血管内皮细胞侵袭性的影响。方法:利用结合CD105单克隆抗体的免疫磁珠内皮细胞分选系统特异性分选出22例高级别胶质瘤微血管内皮细胞(其中8例Ⅲ级胶质瘤,14例Ⅳ级胶质瘤,13例男性和9例女性组成)。应用siRNA技术抑制高级别胶质瘤血管内皮细胞中Stathmin表达,Transwell侵袭实验检测Stathmin基因沉默对高级别胶质瘤血管内皮细胞迁移的影响。结果:采用RNA干扰Stathmin的高级别胶质瘤微血管内皮细胞中Stathmin的mRNA和蛋白表达均较对照组显著下降,而迁移细胞数也显著低于对照组(P<0.01)。结论:通过siRNA抑制高级别胶质瘤血管内皮细胞中Stathmin表达可抑制其迁移。Objective： To investigate the influence of Stathmin gene silencing on the invasion of glioma-derived microvascular en- dothelial cells （GDMEC）. Methods： Microvascular endothelial cells were isolated from 22 cases of human high grade gliomas, and puri- fied by magnetic beads coated with anti-CD105 antibody（including 8 WHO III glioma, 14 WHO IV glioma, 13 male, 9 female）. Stathmin expression was silenced by application of specific siRNA in the high grade GDMEC. The migration of GDMEC were investigated by Transwell. Results： Stathmin gene silencing by RNAi significantly decreased the mRNA and protein expression of Stathmin in the mi- crovascular endothelial cells of high grade GDMEC of glioma（P〈0.01）, moreover, lowered the count of migrated microvascular endothe- lial cells （P〈0.01）. Conclusion： Stathmin gene silencing by RNAi could inhibit the migration of microvascular endothelial cells of high grade GDMEC of glioma.

关 键 词：脑胶质瘤 STATHMIN 胶质瘤微血管内皮细胞 SIRNA 

分 类 号：R739.41[医药卫生—肿瘤]