黄金茶种质资源遗传多样性的AFLP分析  

Genetic Diversity of Tea Germplasm Resource ‘Huangjincha'(Camellia sinensis) Revealed by AFLP Analysis

作　　者：吴扬[1] 邓婷婷[1] 李娟[1] 李银花[1] 刘硕谦[1] 王英姿[1] 黄建安[1] 刘仲华[1] 

机构地区：[1]国家植物功能成分利用工程技术研究中心/湖南农业大学茶学教育部重点实验室,湖南长沙410128

出　　处：《茶叶科学》2013年第6期526-531,共6页Journal of Tea Science

基　　金：国家科技支撑计划(2011BAD01B01);国家自然科学基金(30871572);作物种质创新与资源利用重点实验室科学基金开放项目(12KFXM12)

摘　　要：应用AFLP-银染技术，分析黄金茶群体111个株系的遗传多样性与亲缘关系。选用多态性高、分辨力强的引物组合E37M32、E41M33与E41M42分别扩增样品基因组DNA，共得到229条清晰条带，其中多态性条带186条，多态位点百分比为81．22％，反映了样品基因组DNA具有较高的多态性。111个样品得出的平均有效等位基因数（Ne）为1．42~0．35，平均Nei’S基因多样性（H）为O．25＋0．18，平均Shannon’S信息指数（I）为0．38＋0．25。应用NTSYSpc2．1软件，计算得到111个样品间的相似系数在0．65～0．99之间，平均为0．76。根据UPGMA法，将111个株系分成8大类群，绘制样品AFLP聚类树状图。本研究为黄金茶种质资源的保护及利用，从分子水平提供了一定的依据。111 tea strains from ＇Huangjincha＇ population were analyzed by AFLP-sliver staining protocol for detecting the genetic diversity and relationship. AFLP fingerprinting of 111 samples with primer combinations E37M32, E41M33 and E41M42 revealed a total number of unambiguous 229 bands, of which 186 were polymeric and the polymorphism frequency was 81.22%. The results showed that abundant diversities existed among samples of genomic DNA. The mean of effective number of alleles, Nei＇s gene diversity and Shannon＇s information index of 111 samples were 1.42~：0.35, 0.25＋0.18, 0.38＋0.25 respectively. As analyzed by NTSYSpc2.1, the similarity coefficient of 111 samples ranged from 0.65 to 0.99, and the mean was 0.76. These tea strains were divided into 8 groups by UPGMA based on the mean of similarity coefficient, and AFLP dendrogram was established. In conclusion, the results provide the basis for the protection and utilization of ＇Huangjincha＇ population from molecular level.

关 键 词：黄金茶 AFLP 遗传多样性 

分 类 号：S571.1[农业科学—茶叶生产加工] S324[农业科学—作物学]