绿色木霉β-葡萄糖苷酶基因在大肠杆菌中的克隆  

Cloning of β-glucosidase genes from Tridchoderma viride into E.coli

作　　者：刘颖[1] 韩春然[1] 张帅[1] 张玮玮[1] 窦博鑫[1] 

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150076

出　　处：《食品工业科技》2013年第24期177-180,共4页Science and Technology of Food Industry

基　　金：黑龙江省高校科技创新团队建设计划项目(2010td04);黑龙江省普通高等学校青年学术骨干支持计划项目(160135);黑龙江省自然科学基金项目(D01-24)

摘　　要：以绿色木霉(Trichoderma viride)的总RNA为模板,根据GenBank上检索的β-葡萄糖苷酶基因bglⅠ(M55080)DNA序列,设计特异性引物,进行PT-PCR扩增bglⅠ片段,将其连接到pMD18-T载体并转化到大肠杆菌JM109测序。测序结果表明,所获得的DNA序列与GenBank上检索的β-葡萄糖苷酶基因的核苷酸序列同源性达98.8%。As total RNA of Trichoderma viride was the template. According to the DNA sequence of β- glucosidase genes （bgl Ⅰ） from GenBank,specific primers were designed to amplificate fragments of bgl Ⅰ by PT-PCR,then they were cloned into pMD18-T vector and transformed into E.cofi JM109 to determinate sequence. Sequence analysis indicated that the β-glucosidase genes nucleotide similarity of DNA sequences among Trichoderma viride and others obtained from GenBank was 98.8%.

关 键 词：绿色木霉 Β-葡萄糖苷酶基因 大肠杆菌 克隆 

分 类 号：Q785[生物学—分子生物学]