二甲肼诱导大鼠肠癌过程中增殖细胞核抗原的表达  

Expression of proliferating cell nuclear antigen during the development of rat colon cancer induced by 1,2 dimethylhydrazine

作　　者：吴成秋[1] 陈雯[1] 张桥[1] 钟小明[1] 魏青[1] 吴大伟[1] 

机构地区：[1]中山医科大学公共卫生学院卫生毒理学教研室,广东广州510089

出　　处：《中国癌症杂志》2000年第6期553-555,共3页China Oncology

基　　金：美国中华医学会 (CMB)资助项目! (No.中山医科大学国访970 16 );国家自然科学基金资助项目! (No .396 70 6 44)。

摘　　要：目的 :研究增殖细胞核抗原 (PCNA)在大肠癌发生发展过程中的表达规律以及它们的相关关系。方法 :用免疫组化SABC法检测 1,2 二甲肼成功诱导大鼠肠癌发生发展过程中的PCNA的表达。结果 :用 1,2 二甲肼诱导大鼠肠癌模型 ,诱癌率达 5 7 6 9%。PCNA表达在肠癌诱导的第 5周明显改变 ,到第 15周肠癌形成时达到较高水平 ,并与癌前病变及癌的浸润程度呈正相关。结论 :PCNA在肠癌发生的早期有高表达 ,并与癌前病变及癌的发展呈正相关 。Purpose:To study the expression of proliferating cell nuclear antigen (PCNA) druing the colonic carcinogenesis, and to explore the relationship between the expression of PCNA and carcinogonesis. Methods: Immunohistochemical starning was used to detect the expression of PCNA in the colonic carcinogenesis model induced by 1,2 dimethylhydrazine. Results:The colonic carcinogensis model was successfully established in theis experiment, the cancer inducing rate was 57.69%. The expression of PCNA increased at the 5th week It was higher than that of the control group ( P <0 05), and reached a higher level at the 15th week when the cancer had developed. Meanwhile, there was significant correlation between the level of PCNA expression and the stage of carcinogenesis. Conclusions:The increased expression of PCNA appears in the early stage of carcinogenesis, and is related to development of preneoplastic lession and cancer. PCNA may be a good biomarker for carcinogenesis.

关 键 词：大肠癌 增殖细胞核抗原 二甲肼 动物模型 

分 类 号：R735.34[医药卫生—肿瘤]