Ketanserin improves cardiac performance after myocardial infarction in spontaneously hypertensive rats partially through restoration of baroreflex function  

作　　者：Jian-guang YU En-hui ZHANG Ai-jun LIU Jian-guo LIU Guo-jun CAI Ding-feng SU 

机构地区：[1]Department of Pharmacology, Second Military Medical University, Shanghai 200433, China

出　　处：《Acta Pharmacologica Sinica》2013年第12期1508-1514,共7页中国药理学报（英文版）

摘　　要：Aim： Baroreflex dysfunction is associated with a higher rate of sudden death after myocardial infarction （MI）. Ketanserin enhances baroreflex function in rats. The present work was designed to examine whether ketanserin improves the post-MI cardiac function and to explore the possible mechanism involved. Methods： Spontaneously hypertensive rats （SHR） were treated with ketanserin （0.3 mg·k-1·d-1）. Two weeks later, blood pressure and baroreflex function were measured, followed by a ligation of the left coronary artery. The expressions of vesicular acetylcholine transporter （VAChT） and a7 nicotinic acetylcholine receptor （a7-nAChR） in ischemic myocardium, angiogenesis, cardiac function, and left ventricular （LV） remodeling were evaluated subsequently. Results： Ketanserin significantly improved baroreflex sensitivity （0.62＋0.21 vs 0.34＋0.12 ms/mmHg, P〈O.01） and vagal tonic activ- ity （heart rate changes in response to atropine, 54.8＋16.2 vs 37.6＋13.4 bpm, P〈O.01） without affecting the blood pressure or basic heart rate in SHR. Treatment of SHR with ketanserin prominently improved cardiac function and alleviated LV remodeling, as reflected by increases in the ejection fraction, fractional shortening, and LV systolic pressure as well as decreases in LV internal diameter and LV relative weight. The capillary density, vascular endothelial growth factor expression, and blood flow in the ischemic myocardium were significantly higher in the ketanserin-treated group. In addition, ketanserin markedly increased the expression of VAChT and （x7-nAChR in ischemic myocardium. Conclusion： Ketanserin improved post-MI cardiac function and angiogenesis in ischemic myocardium. The findings provide a mechanistic basis for restoring baroreflex function using ketanserin in the treatment of Ml.Aim： Baroreflex dysfunction is associated with a higher rate of sudden death after myocardial infarction （MI）. Ketanserin enhances baroreflex function in rats. The present work was designed to examine whether ketanserin improves the post-MI cardiac function and to explore the possible mechanism involved. Methods： Spontaneously hypertensive rats （SHR） were treated with ketanserin （0.3 mg·k-1·d-1）. Two weeks later, blood pressure and baroreflex function were measured, followed by a ligation of the left coronary artery. The expressions of vesicular acetylcholine transporter （VAChT） and a7 nicotinic acetylcholine receptor （a7-nAChR） in ischemic myocardium, angiogenesis, cardiac function, and left ventricular （LV） remodeling were evaluated subsequently. Results： Ketanserin significantly improved baroreflex sensitivity （0.62＋0.21 vs 0.34＋0.12 ms/mmHg, P〈O.01） and vagal tonic activ- ity （heart rate changes in response to atropine, 54.8＋16.2 vs 37.6＋13.4 bpm, P〈O.01） without affecting the blood pressure or basic heart rate in SHR. Treatment of SHR with ketanserin prominently improved cardiac function and alleviated LV remodeling, as reflected by increases in the ejection fraction, fractional shortening, and LV systolic pressure as well as decreases in LV internal diameter and LV relative weight. The capillary density, vascular endothelial growth factor expression, and blood flow in the ischemic myocardium were significantly higher in the ketanserin-treated group. In addition, ketanserin markedly increased the expression of VAChT and （x7-nAChR in ischemic myocardium. Conclusion： Ketanserin improved post-MI cardiac function and angiogenesis in ischemic myocardium. The findings provide a mechanistic basis for restoring baroreflex function using ketanserin in the treatment of Ml.

关 键 词：KETANSERIN myocardial infarction BAROREFLEX ANGIOGENESIS a7-nAChR 

分 类 号：Q463[生物学—生理学] Q813.3