无机溶剂法制备β-环糊精的工艺研究  

Preparation technology of β-cyclodextrins with inorganic solvent method

作　　者：王超凡[1,2,3,4,5] 宋拓[1,2,3,4,5] 丁俊美 于婷婷[1,2,3,4,5] 李俊俊 唐湘华[1,2,3,4,5] 谢振荣 张学林[1,2,3,4,5] 高光敏[1,2,3,4,5] 黄遵锡 

机构地区：[1]生物能源持续开发利用教育部工程研究中心 [2]云南省生物质能与环境生物技术重点实验室 [3]云南省酶工程技术研究中心 [4]酶资源开发与应用国家地方联合工程研究中心 [5]云南师范大学生命科学学院,云南昆明650500

出　　处：《中国酿造》2013年第11期112-117,共6页China Brewing

基　　金：国家科技支撑计划课题(2013BAD10B01);国家自然科学基金地区科学基金项目(31160229)

摘　　要：对一种无机溶剂酶催化生产β-环糊精的工艺进行研究,并采用响应面实验法确定了生产β-环糊精的适宜工艺条件,为其开发研究应用提供参考。实验结果表明,生产β-环糊精的最佳条件是选取木薯淀粉作为底物,不经过预处理,环状糊精葡萄糖基转移酶添加量是4U/g,CaCl2的浓度为30mmol/L,pH7.88,温度63.67℃,反应时间17.24 h,产率可达24.02%。生产工艺和传统工艺相比,产率明显增高,提高了生产效率。A preparation technology of β-cyclodextrins with inorganic solvent was studied. The response surface experimental method made a suitable process conditions for productions of β-cyclodextrin and provided a reference for the development of research and application. The production results showed that the optimal production conditions were as follow： substrate tapioca starch without pretreatment, the amount of enzyme 4U/g, the concentration of CaCl2 30mmol/L, pH value 7.88, temperature 63.67℃, reaction time 17.24h. Under these conditions, yield was up to 24.02%. The production technology greatly shortens the reaction time compared with traditional technologies significantly improves yield and promotes the preparation efficiency.

关 键 词：环状糊精葡萄糖基转移酶 无机溶剂法 Β-环糊精 生产工艺 

分 类 号：Q55[生物学—生物化学]