蛋黄卵磷脂提取方法的研究  

Study on the extraction methods of egg yolk lecithin

作　　者：刘颖[1] 肖静静[1] 李硕绵[1] 陈立功[1] 王丽叶[1] 王迎春[1] 董世山[1] 

机构地区：[1]河北农业大学动物医学院/河北省兽用生物制品工程技术研究中心,河北保定071001

出　　处：《河北农业大学学报》2013年第6期105-108,共4页Journal of Hebei Agricultural University

基　　金：河北省科技支撑计划项目(07220401D-01)

摘　　要：本试验比较了乙醇提取、先乙醇后丙酮提取和先丙酮后乙醇提取3种蛋黄卵磷脂提取方法,以期获得一种最优的提取方法。同时,在各提取方法中分别采用不同乙醇体积分数观察其对蛋黄卵磷脂提取效果的影响。试验采用钼黄比色法测定卵磷脂的含量;通过薄层色谱法(TLC)定性检测蛋黄卵磷脂。结果表明:先利用95%的乙醇处理蛋黄,然后用丙酮脱油为最优蛋黄卵磷脂提取方法,卵磷脂的提取率、纯度和含量都较高,分别为8.6%,80.3%,6.9%。提取得到的蛋黄卵磷脂薄层色谱图和卵磷脂标准品的薄层色基本谱图一致。The aim of this study was to explore the best extraction method of egg yolk lecithin. The egg yolk was treated by ethanol, ethanol-acetone and acetone-ethanol organic solvent,and adopted different, ethanol volume fraction. The lecithin amount was measured by molybdenum- yellow-spectrophotometry method, and the property of lecithin was tested by thin-layer chro- matography（TLC） method. The results are as follows： The best procedure of egg yolk lecithin extraction was that dissolving the yolk with ethanol at first, and then deoiling with acetone. The extraction yield , lecithin purity and amount were 8.6 %,80.3 %, 6.9 %,respectively. The TLC chrom_atogram of extractive lecithin were as same as those of lecithin standard sample.

关 键 词：蛋黄 卵磷脂 薄层色谱 提取 

分 类 号：S132[农业科学—农业基础科学]