17β-雌二醇和跑台运动对去卵巢大鼠骨组织及子宫ERα蛋白表达影响的对比研究  

Comparative Study of 17beta-Estradiol and Exercise on Bone and Uterine ERα Protein Expression in Ovariectomized Rats

作　　者：文思敏[1] 卜淑敏[1] 许晖[1] 段晓丽[1] 司会群[1] 

机构地区：[1]首都体育学院,北京100191

出　　处：《现代生物医学进展》2013年第33期6479-6483,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30771046);北京市教委科技创新平台项目(PXM2012-014206-000051)

摘　　要：目的:雌激素受体α(ERα)作为雌激素调节骨效应的主要受体在雌激素对骨量和骨代谢的调节中发挥重要的作用,为此本研究比较了17β-雌二醇(E2)和跑台运动对去卵巢大鼠骨组织和子宫ERα蛋白表达影响的异同。方法:将40只健康3月龄雌性SD大鼠,按体重分层后随机分为假手术、去卵巢、雌激素和运动四个组。手术1周后,雌激素组大鼠每周按体重颈部皮下注射三次17β-雌二醇,每次25μg/kg体重。运动组每周进行4次45 min、速度18 m/min、坡度5°的跑台训练。连续给药或运动处理14周后,采用放射免疫法和免疫组织化学法分别检测血清E2水平以及胫骨和子宫ERα蛋白表达的变化。结果:大鼠去卵巢后,子宫重量、子宫重量指数和血清E2水平显著下降,补充外源性17β-雌二醇后,三项指标均显著增加;但运动处理只能增加血清E2水平,对子宫重量和子宫重量指数均无显著影响。子宫ERα蛋白免疫组化结果显示:ERα在去卵巢组子宫内膜腔上皮、腺上皮及基质中的表达显著低于假手术组,而在雌激素组中的表达高于去卵巢组,运动组各部位ERα表达虽有所增加,但是增加的程度远低于雌激素组。胫骨近端ERα蛋白免疫组化结果显示:去卵巢后,胫骨近端骨骺端软骨细胞的细胞核内ERα表达减少,运动和雌激素干预后,胫骨近端ERα表达增加。结论:运动和雌激素处理均能刺激去卵巢大鼠子宫和骨组织ERα蛋白的表达,但运动处理无雌激素处理增加子宫重量的副作用。可见在绝经后骨质疏松的预防中,运动处理可能要优于雌激素处理。Objective： As a major receptor of estrogen effect, estrogen receptor alpha （ERα） play an important role in regulation of bone mass and bone metabolism. Hence this paper compare the similarities and differences of 17β-estradiol （E2） and exercise on ERα protein expression in bone and uterine of ovariectomized rat. Methods： 40 healthy 3-month-old female Sprague-Dawley rats were randomly divided into four groups, namely sham group （Sham）, ovariectomized group （OVX）, estrogen group （E2） and exercise group （EX）. One week after surgery, the rats in E2 group were injected subcutaneously with E2 three times a week at the concentration of 50 μg/kg. The rats in EX group were trainied four times per week at speed of 18 m/min for 45 min/time. After treatment for 14 weeks, the serum level of E2 were tested by radioimmunoassay and the protein expressions of ERoL in both bone and uterine were detected by immunohistochemistry and western blotting. Results： The results indicated that there were a decrease in uterine weight, uterus weight index, and serum E2 level after OVX surgery and all the parameters were reversed after supplemented with E2. There were no significant differences in uterine weight and uterine weight index between OVX group and EX group, but significant difference was seen in serum level of E2. The results of immunohistochemistry showed that the ERa immunoreactivity in uterus endometrial, glandular epithelium, and stromal of the OVX rats was significantly lower than that of in Sham rats. The ERa immunoreactivity of the uterus endometrial and glandular epithelium in both E2 and EX group was significantly lower than that of in OVX group, but the increase degree of EX group was much lower than that of in E2 group. The immunohistochemistry results of proximal tibia showed that ERα immunoreactivity in nuclei of epiphyseal cartilage cells was lower in OVX group than that of in the other three groups. Conclusion： These results indicated that both exercise and E2 treatment could stimu

关 键 词：17Β-雌二醇 运动 去卵巢大鼠 ERΑ 绝经后骨质疏松 

分 类 号：Q95-3[生物学—动物学] R68[医药卫生—骨科学]