肝硬化患者肠道微生物代谢功能的宏基因组学研究  

Metabolism and function of intestinal microbiota from liver cirrhosis patients: a metagenomic study

作　　者：魏晓[1] 邹大阳[1] 闫夏贝 杨展[1] 崔茜[1] 王思淼[1] 黄留玉[1] 袁静[1] 

机构地区：[1]军事医学科学院疾病预防控制所,北京100071

出　　处：《军事医学》2013年第11期801-807,共7页Military Medical Sciences

基　　金：国家自然科学基金资助项目(81071321)

摘　　要：目的分析肝硬化患者远端肠道微生态菌群代谢功能的变化。方法选取16例肝硬化患者及20例正常人,提取其肠道微生物宏基因组DNA进行高通量Solexa测序,对测序基因进行代谢功能的注释,比较肝硬化患者与正常人之间的差异,找出疾病相关的肠道微生物代谢功能的变化。结果肝硬化患者肠道菌群功能多样性降低,对药物、必需氨基酸、丙酸盐等的代谢能力以及炎性反应显著增强,而对丁酸盐、胆汁酸的代谢能力以及细胞周期相关的功能显著降低。结论在肝硬化的影响下,肠道微生物的生长环境被破坏,肠道菌群为了适应环境,在功能和代谢方面表现出一定程度的代偿。Objective To investigate the metabolism and function of the intestinal microbiota from liver cirrhosis patients.Methods Sixteen cases of liver cirrhosis and twenty normal individuals were selected , whose intestinal microbiota metagenomic DNA was extracted , followed by high-throughput Solexa sequencing and the bioinformatics analysis of metabo-lism and function annotation to compare the differences between the patients and normal subjects and find out about the cir -rhosis-related functions .Results The functional diversity was significantly reduced in the intestinal microbiota of cirrhotic patients.At the module or pathway level , the intestinal microbiota of patients showed an enrichment in metabolisms of drugs, essential amino acid , propanoate metabolism and inflammatory reaction , whereas an opposite tendency was observed in the metabolic ability of butyrate , bile acid and cell cycle .Conclusion Under the influence of liver cirrhosis , the growth environment in the intestine is destroyed , causing, the intestinal microbiota the exhibit some compensation to adapt to the changed intestinal micro-environment .

关 键 词：肝硬化 宏基因组学 谷胱甘肽 必需氨基酸 胆汁酸 

分 类 号：R575.2[医药卫生—消化系统]