辐照诱导人正常肝细胞系HL-7702细胞远后的微卫星不稳定性  

Radiation-induced Delayed Microsatellite Instability in Human Normal Liver HL-7702 Cells

作　　者：赵婷[1,2,3] 叶飞[1,2,3] 贺鹏博[1,2,3] 刘雄雄[1,2,3] 李强[1,2] 

机构地区：[1]中国科学院近代物理研究所,甘肃兰州730000 [2]中国科学院重离子束辐射生物医学重点实验室,甘肃兰州730000 [3]中国科学院大学,北京100049

出　　处：《原子核物理评论》2013年第4期494-502,共9页Nuclear Physics Review

基　　金：国家重点基础研究发展规划项目(973计划)(2010CB834203);国家自然科学基金委员会-中国科学院大科学装置联合基金资助项目(U1232207);国家自然科学基金资助项目(10905080;11075191)~~

摘　　要：采用高传能线密度(LET)的12C6+离子束和低LET的X射线辐照人正常肝细胞系HL-7702细胞,利用微卫星不稳定性(MSI)检测来分析直接受照射细胞和通过转移培养基方式旁细胞传代八代子细胞以MSI表征的远后效应。实验结果表明,12C6+离子束诱导的远后效应较X射线的低;旁细胞的远后效应较直接受照射细胞的高;辐射引起的MSI与杂合性丢失(LOH)的发生率具有位点特异性。结果提示,重离子放射治疗较X射线放射治疗对正常组织引发的辐射风险要小,可通过对MSI高发位点的筛选来评估放疗后患者长期生存状况和二次癌症发生风险。Human normal liver cell line HL-7702 cells were irradiated with high linear energy transfer （LET） 12C6＋ ions and low-LET X-rays, respectively. Delayed effect in terms of microsatellite instability （MSI） in progenies of the directly irradiated cells and bystander ceils, obtained in the way of medium transfer at the 8th passage postirradiation, were examined. The delayed effect induced by the high-LET 12C6＋ ions was different from that induced by the low-LET X-rays, and a higher incidence of MSI was observed in the progenies of the cells after exposure to the X-rays than to the 12C6＋ ions. We also found that the delayed effect in the progenies of the bystander cells was much more severe than those of directly irradiated cells. Furthermore, the events of MSI and loss of heterozygosity （LOH） induced by the ionizing radiations were not randomly distributed throughout the genorne and specific loci existed indeed. These results imply that the radiation risk to normal tissues is lower in heavy ion therapy than in conventional X-ray radiotherapy, and the analysis of microsatellite loci with MSI high frequency occurrence can be applied to access long-term survival condition and second cancer risk for the patients after radiotherapy.

关 键 词：重离子 放射治疗 远后效应 微卫星不稳定性 杂合性丢失 

分 类 号：R730[医药卫生—肿瘤] Q691[医药卫生—临床医学]