大豆抗原蛋白Glycinin对鲤稚鱼和幼鱼血液主要生化指标的影响  

Effects of Soybean Antigen Glycinin on Blood Biochemical Parameters in Larval and Juvenile Common Carp, Cyprinus carpio

作　　者：赖红娥[1] 吴莉芳[1] 秦贵信[1] 张东鸣[1] 赵晗[1] 王洪鹤[1] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118

出　　处：《吉林农业大学学报》2013年第6期736-741,共6页Journal of Jilin Agricultural University

基　　金：吉林省自然科学基金项目(20101577)

摘　　要：以初始体质量鲤稚鱼(10.12±0.08)g和鲤幼鱼(116.89±0.13)g为研究对象,利用分离纯化的大豆抗原蛋白Glycinin不同比例(0,3.0%,6.0%,9.0%,12.0%)添加在配合饲料中,以鱼粉为动物蛋白源,糊精、鱼油、玉米油等为能源、纤维素为填充物配制成5种等氮(鲤稚鱼40%,鲤幼鱼36%)、等能(鲤稚鱼16.9 MJ/kg,鲤幼鱼15.2 MJ/kg)的半精制配合饲料,进行为期8周的饲养试验。饲养试验结束后,采用BS400全自动生化分析仪对血液主要生化指标进行测定。结果表明:大豆抗原蛋白Glycinin对鲤稚鱼血液中尿素氮、胆固醇、甘油三酯、血糖、总蛋白、白蛋白、球蛋白、谷草转氨酶、谷丙转氨酶的含量影响不显著(P>0.05)。大豆抗原蛋白Glycinin对鲤幼鱼血液中血糖、总蛋白、白蛋白、球蛋白、谷草转氨酶、谷丙转氨酶的影响不显著(P>0.05),但血液中的尿素氮、胆固醇、甘油三酯的含量随着大豆抗原蛋白Glycinin添加量的增加呈下降趋势,添加量为12.0%组的尿素氮、胆固醇、甘油三酯的含量显著低于对照组(P<0.05)。The trial was carried out on larval and juvenile common carp with the initial weight （ 10.12 ± 0.08） g and （116.89 ± 0.13） g for eight weeks. Five semi-refined diets of isonitrogenous （larval common carp 40%; juvenile common carp 36% ） and isocaloric（larval common carp 16.9 MJ/kg; juvenile com- mon carp 15.2 MJ/kg） were formulated with different ratios of purified soybean antigen Glycinin（0, 3.0% ,6.0% ,9.0%, 12.0% ） and fish meal as animal protein, dextrin, fish oil, corn oil as energy, cellulose as filler. The blood biochemical parameters were tested using the BS400 automatic biochemistry analyzer after the feeding trials. The results show that the soybean antigen Glycinin had no significant （P 〉 0.05） influence on the concentration of urinary nitrogen, cholesterol, triglyeride, serum glucose, total protein, albumin, globin, glutamic oxalacetic transaminase and glutamic pyruvic transaminase of lar- val common carp. The soybean antigen glycinin had no significant （ P 〉 0. 05） influence on the concen- tration of serum glucose, total protein, albumin, globin, glutamic oxalacetic transaminase and glutamic pyruvic transaminase of juvenile common carp; but with the increase of replacement ratio of glycinin, urinary nitrogen, cholesterol, triglyeride in blood of juvenile carp showed a declining trend and it signifi- cantly decreased（ P 〈 0.05） in 12.0% groups of juvenile common carp.

关 键 词：GLYCININ 鲤 稚鱼 幼鱼 血液生化指标 

分 类 号：S963[农业科学—水产养殖] S965.116[农业科学—水产科学]