原发性膀胱移行细胞癌p16基因缺失突变的研究  

Study of p16 gene loss and mutation in primary transitional cell carcinomas of urinary bladder

作　　者：杨志伟[1] 郑新民[1] 胡礼泉[1] 李世文[1] 

机构地区：[1]武汉大学中南医院泌尿外科,430071

出　　处：《中华实验外科杂志》2001年第1期8-9,共2页Chinese Journal of Experimental Surgery

摘　　要：目的　探讨 p16基因在原发性膀胱移行细胞癌中的改变。方法　应用多聚酶链(PCR)和多聚酶链 单链构象多态性 (PCR SSCP)方法对 38例膀胱移行细胞癌标本p16基因的缺失、突变进行分析。结果　原发性膀胱移行细胞癌 p16基因缺失率为 18.42 % ,未观察到突变。缺失、突变率明显低于培养的膀胱癌细胞系。结论　肿瘤细胞自原体转入培养环境的适应阶段p16基因缺失或突变可能有助于肿瘤细胞的增殖 。Objective To explore the alteration of p16 gene in primary transitional cell carcinomas(TCCs) of bladder. Methods PCR and PCR SSCP methods were used to detect the loss and mutation of p16 gene in 38 specimens of primary TCCs of urinary bladder. Results The loss rate of p16 gene was 18.42% in 38 TCCs specimens and the mutation of p16 gene was not observed. Conclusion There was an apparently lower frequency of p16 loss and mutation in primary bladder cancer compared with the culture tumor counterparts. The loss of p16 gene confers a selective growth advantage during adaptation of tumor cells to culture and hence is probably important in the generation of bladder cancer cell lines. [

关 键 词：P16基因 基因缺失 基因突变 原发性膀胱移行细胞癌 

分 类 号：R737.14[医药卫生—肿瘤] R730.23[医药卫生—临床医学]