负压空化法提取银杏叶中原花青素及其分离纯化  

Negative Pressure Cavitation-Assisted Extraction and Purification of Proanthocyaidins from Ginkgo biloba Leaves

作　　者：王燕芹[1] 徐莉[1,2] 曹福亮[3] 王未凡[1] 赵俸艺 杨世龙[1] 雷萌[1] 陈泳[1] 唐颖[2] 蒋国斌[1] 汪贵斌[3] 

机构地区：[1]南京林业大学理学院,江苏南京210037 [2]南京林业大学现代分析测试中心,江苏南京210037 [3]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《食品科学》2013年第24期6-10,共5页Food Science

基　　金：国家林业公益性行业科研专项(20120460102;201004015);江苏省高校"青蓝工程"培养对象资助项目;2012年南京林业大学大学生实验室创新项目

摘　　要：采用负压空化法研究银杏叶中原花青素的提取工艺。单因素试验考察提取压力、乙醇体积分数、料液比、提取时间、提取次数对原花青素提取率的影响。利用响应面分析法优化负压空化法提取银杏叶中原花青素的工艺条件。结果表明:提取压力-0.05MPa、料液比1:10.1(g/mL)、乙醇体积分数50%时,原花青素的提取率可达5.54%。采用大孔树脂法对原花青素粗提液进行分离纯化,通过静态吸附和动态解吸实验筛选出AB-8大孔树脂具有最佳效果,在30℃条件下吸附10h,吸附量为8.74mg/g,用无水乙醇以2mL/min的流速进行洗脱时,AB-8对原花青素的解吸率为80.5%。This study dealt with the optimization of negative pressure cavitation-assisted extraction of proanthocyandins from Ginkgo biloba leaves. Using the extraction efficiency of proanthocyandins as the response value, response surface and contour plots were drawn, and the optimum extraction conditions were determined as follows： extraction pressure of --0.05 MPa, solid-to-liquid ratio of 1：10.1 （g/mL） and ethanol concentraction of 50%. Under these extraction conditions, the extraction efficiency of proanthocyandins was 5.54%. The crude extract of proanthocyanidins was purified with macroporous adsorption resin. Our static adsorption and dynamic desorption experiments indicated that AB-8 was the best resin for proanthocyandin purification, and the optimum chromatographic conditions were found as follows： 10 h adsorption at 30 ℃, resulting in an adsorption quantity of 8.74 mg/g, and desorption with absolute ethanol at a flow rate of 2 mL/min, providing a desorption efficiency of 80.5%.

关 键 词：银杏叶 原花青素 负压空化 提取 纯化 

分 类 号：TS201.1[轻工技术与工程—食品科学] Q946.49[轻工技术与工程—食品科学与工程]