我国沿海主要经济贝类中典型人类肠道病毒的污染分布  

Contamination of typical human enteric viruses in economic shellfish along the Chinese coast

作　　者：明红霞[1] 樊景凤[1] 吴利军[1,2] 岳龙涛[3] 高雪琴[3] 梁玉波[1] 

机构地区：[1]国家海洋环境监测中心,辽宁大连116023 [2]中国科学院武汉病毒研究所,湖北武汉430071 [3]山东省医药生物技术研究中心、卫生部生物技术药物重点实验室,山东济南250062

出　　处：《微生物学报》2014年第1期69-79,共11页Acta Microbiologica Sinica

基　　金：国家“863计划”(2006AA09Z162,2006AA09Z170);国家专项(908-01-ZH3);海洋公益性行业科研专项(201105007);国家海洋局近岸海域生态环境重点实验室开放研究基金(201201)~~

摘　　要：【目的】了解我国沿海主要经济贝类中肠道病毒的污染分布对保障贝类食用安全具有重要作用。【方法】通过建立5种典型人类肠道病毒的特异、灵敏、高通量的基因芯片检测技术,开展全国范围内沿海主要海水增养殖区经济贝类体内肠道病毒的污染调查。【结果】采集的162份经济贝类中肠道病毒的阳性检出率分别为:甲肝病毒4.3%;诺如病毒14.8%;轮状病毒6.2%;星状病毒5.6%;腺病毒9.9%,其中诺如病毒污染居于首位,该检测结果与PCR结果高度一致。我国沿海省市主要养殖区内贝类均受到了肠道病毒不同程度的污染,且不同省市之间差异不显著。在选取的6类经济贝类中,牡蛎中肠道病毒阳性检出率最高,其次是毛蚶和泥蚶。【结论】本研究表明,在我国,沿海主要经济贝类受到肠道病毒污染是一种较普遍的现象,给人类健康带来了潜在的危害,本研究为贝类食品的安全监控提供了基础数据资料。[Objective]The objective of this study is to understand the contamination of human enteric viruses in economic shellfish along the Chinese coast,an important issue of ensuring the seafood safety.[Methods]We established the specific,sensitive and high-throughput gene chip technology,to investigate the contamination of economic shellfish by enteric viruses across a large geographical region of China.[Results]The percentage of positive samples for each virus was as follows： Hepatitis A Virus 4.3%,norovirus 14.8%,rotavirus 6.2%,astrovirus 5.6%,and adenovirus 9.9%.In these five viruses,norovirus was contaminated in the first place.The results detected by gene chip were highly consistent with that of polymerase chain reaction （ PCR）.The economic shellfishes in shellfish-growing areas along the coastal cities were all contaminated with enteric viruses at different levels. However,there was no significant correlation between any two cities.In the selected 6 economic shellfishes,oyster had the highest positive rate of enteric viruses,followed by blood clam.[Conclusion]The contamination of shellfish with human enteric viruses was common across the main coastal cities of China,indicating a potential public health threat from seafood.

关 键 词：基因芯片 人类肠道病毒 沿海经济贝类 

分 类 号：X172[环境科学与工程—环境科学]