乳杆菌S-层蛋白质的多样性及其潜在应用  

Heterogeneity of surface layer proteins in Lactobacillus and their potential applications

作　　者：孙芝兰[1,2] 胡淑敏[2,3] 孔健[2] 

机构地区：[1]江苏省农业科学院农业生物技术研究所,江苏省农业生物学重点实验室,江苏南京210014 [2]山东大学微生物技术国家重点实验室,山东济南250100 [3]青岛啤酒股份有限公司,啤酒生物发酵工程国家重点实验室,山东青岛266000

出　　处：《江苏农业学报》2013年第6期1489-1495,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家科技支撑计划项目(2012BAD39B01);国家“863”项目(2011AA100902);江苏省农业科技自主创新基金项目[CX(12)3041];江苏省自然科学基金项目(BK20130712)

摘　　要：S层(Surface layer)是存在于细菌及古细菌细胞外由蛋白质或糖蛋白质亚单位组成的单分子层晶格状结构。乳杆菌属中也发现了S-层蛋白质的存在,分子量介于2.5×104至7.1×104之间,是已知最小的一类S-层蛋白质,其蛋白质结构、功能与其他细菌的S-层蛋白质存在较大差异。乳杆菌S-层蛋白质独特的空间结构、自组装功能及细胞壁锚定功能使其在纳米生物技术、活载体疫苗开发、诊断学、新的生物催化剂领域具有很强的应用潜力。本文重点讨论乳杆菌S-层蛋白质的编码基因、蛋白质结构、与宿主菌结合方式及功能的多样性,并论述其潜在的应用前景。Surface layers （S-layers） are monomolecular crystalline arrays composed of protein or glycoprotein sub- units present as the outermost component of cell wall in bacteria and archaea. Several Lactobacillus strains possess single S- layer with molecular masses ranging from 2.5 x 104 to 7.1 x 104. The typical characteristics distinguishing from other S-layer proteins were small size, diversity, and mediating the adhesion to different host tissues. The unique features of Lactobacillus S-layer proteins include their high degree of spatial structure, self-assembly properties and cell wall binding function, mak- ing them have the potential application in nanobiotechnology, live carrier vaccine development, diagnostics and biocatalyst. In this paper, the diversity of S-layer-protein genes, S-layer protein structure and the cell wall binding patterns as well as the potential application were reviewed.

关 键 词：乳杆菌 S-层蛋白质 晶格状结构 多样性 

分 类 号：Q939.117[生物学—微生物学]