一种快速定量检测益生菌制品中植物乳杆菌的方法  

A rapid quantitative detection method of Lactobacillus plantarum in the probiotics products

作　　者：陈臣[1] 任婧[1] 周方方[1] 刘振民[1] 郭本恒[1] 

机构地区：[1]光明乳业股份有限公司研究院乳业生物技术国家重点实验室,上海200436

出　　处：《中国微生态学杂志》2013年第12期1439-1443,1450,共6页Chinese Journal of Microecology

基　　金：科技部"十二五"国家科技支撑计划课题:发酵乳制品乳酸菌菌种与发酵剂的研究与开发(2013BAD18B01);科技部"十二五"国家863课题:优良益生菌高效筛选与应用关键技术(2011AA100901)

摘　　要：目的建立快速定量检测植物乳杆菌的方法，排除近缘菌的干扰。方法利用SMM系统筛选植物乳杆菌种特异序列，根据特异序列设计引物进行实时荧光定量PCR反应。结果设计的引物具有良好的种特异性，检测限为2．26×10^2CFU／mL。结论该方法快速灵敏，可以在实际生产中应用。Objective To establish a specific method for the rapid and quantitative detection of Lactobacillus plantarum without any interference. Methods SMM system was used to identify L. plantarum specific sequences. The primers were chosen according the specific sequences to develop a real-time PCR assay. Results The designed primers had good specificity for L. plantarum without interference from L. paraplantarum and L. pentosus and the detection limit of the method was 2.26 ×10^2 CFU/mL. Conclusion The method was rapid and accurate and could be used in actual production.

关 键 词：植物乳杆菌 SMM系统 实时荧光定量PCR 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]