多基因甲基化检测对结直肠癌早期诊断的意义  

Significance of multiple gene methylation detection in early diagnosis of colorectal cancer

作　　者：王秀江[1] 卢润章[2] 张肖洁[1] 李春明[1] 杨光[1] 韩艳[1] 田菲[1] 修崇坤[1] 赵经慧[2] 

机构地区：[1]哈尔滨市第一医院消化科,黑龙江哈尔滨150010 [2]哈尔滨血液病肿瘤研究所

出　　处：《胃肠病学和肝病学杂志》2013年第12期1228-1230,共3页Chinese Journal of Gastroenterology and Hepatology

基　　金：哈尔滨市科技创新人才研究专项资金项目(2011RFXYS049)

摘　　要：目的通过对结直肠癌(CRC)组织中多基因甲基化水平的检测,探讨多基因甲基化对CRC早期诊断的意义。方法选取25例CRC患者胃镜采集标本组织,通过甲基化特异性PCR(MSP)检测CRC和炎性组织中甲基化水平,分析其与CRC患者临床各指标的关系。结果在CRC患者中,APC、P16、MLH1、DCC甲基化率分别为52.0%(13/25)、32.0%(8/25)、44.0%(11/25)、60.0%(15/25)。炎性组织中仅有1例DCC甲基化异常表达,甲基化率为10.0%(1/10),甲基化阳性率显著高于炎性组织(P<0.05),4种基因甲基化联合检测诊断CRC的阳性率为92.0%(23/25),高于单个基因检测的甲基化阳性率,差异有统计学意义(P<0.01)。结论 APC、P16、MLH1、DCC基因在CRC患者中甲基化水平异常表达,联合检测APC、P16、MLH1、DCC可能作为CRC早期诊断的标志物。Objective To investigate the roles of the methylation status of multiple related genes （APC, P16, MLH1, DCC） in the early diagnosis of colorectal cancer （CRC）. Methods The methylation status of multiple related genes in CRC tissues and inflammatory tissues were detected by methylation specific PCR （MSP）. The relationship of methylation status with clinical features of CRC was analyzed. Results It was demonstrated that the methylation rates of APC, P16, MLH1 and DCC were 52.0% （13/25）, 32.0% （8/25）, 44.0% （11/25）, 60.0% （15/25） in CRC tis- sues, and 10.0% （1/10） in inflammatory tissues, respectively. The methylation of four genes in CRC tissues were more frequently found in cancer tissues than inflammatory tissues （P 〈 0.05）. The diagnostic sensitively by combining four methylation markers was 92.0% in CRC, which was higher than the sensitivity using single testing （P 〈0.01 ）. Conclusion The aberrant DNA methylation expression levels of APC, P16, MLH1 and DCC genes in CRC may relate to pathogenesis. It may be potential that combined detection of APC, P16, MLH1 and DCC provides biomarkers as early di- agnosis of CRC.

关 键 词：结直肠癌 甲基化 APC基因 P16基因 MLH1基因 DCC基因 

分 类 号：R735.3[医药卫生—肿瘤]