抗坏血酸分子印迹电化学传感器的研制与应用  

Development and Application of Ascorbic Acid Molecularly Imprinted Electrochemical Sensors

作　　者：张威[1] 赵晓娟[1] 黄丹丹[1] 陈海光[1] 侯向昶 

机构地区：[1]仲恺农业工程学院轻工食品学院,广东广州510225 [2]广州市质量监督检测研究院,广东广州510110

出　　处：《分析测试学报》2013年第12期1438-1442,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(21005091);广东省教育厅高等学校科技创新项目(2012KJCX0068);广东省质量技术监督局科技项目(2010CZ06)

摘　　要：以抗坏血酸(AA)为模板分子,邻苯二胺(o-PD)为功能单体,采用循环伏安法(CV)在玻碳电极表面电聚合形成聚邻苯二胺(PPD)膜,经水将模板分子洗脱后,制得抗坏血酸分子印迹膜电极。优化的分子印迹条件为:以0.20 mol/L HAc-NaAc(pH 5.2)缓冲溶液为反应介质,o-PD和AA的摩尔比为1∶2,扫描电位为0~0.8 V,扫描速度为50 mV/s。利用CV、方波伏安法(SWV)和电流-时间曲线法(I^t)对该分子印迹传感器的电化学性能进行评价。该传感器的响应电流与AA浓度在低浓度区(1.0×10-6~1.0×10-3mol/L)和高浓度区(1.0×10-3~4.5×10-3mol/L)分别呈良好的线性关系,检出限为4.9×10-7mol/L。运用建立的方法对市售橙汁中的AA进行测定,并用2,6-二氯酚靛酚滴定法进行验证,结果表明,该传感检测技术快速、准确、成本低,适用于橙汁等食品中AA的测定。In order to establish a rapid detection technique of ascorbic acid（ AA）,with AA as template molecule and o-phenylenediamine（ o-PD） as functional monomer,poly（ o-phenylenediamine）（ PPD） film was electropolymerized on the surface of a glassy carbon electrode using cyclic voltammetry（ CV）. Furthermore,the ultrapure water was used to elute the template molecules. The experimental conditions for the electropolymerization reaction were optimized and listed as follows： reaction medium： 0. 20 mol / L HAc- NaAc buffer（ pH 5. 2）,molar ratio of o-PD to AA： 1∶2,potential range： 0-0.8 V,scan rate： 50 mV·s-1. The electrochemical properties of the molecularly imprinted sensor were investigated using CV,square wave voltammetry（ SWV） and current-time curve（ I- t） methods. The response current of the sensor had good linear relationships with concentration of AA in the ranges of 1. 0 × 10- 6- 1. 0 × 10- 3mol / L and 1. 0 × 10- 3- 4. 5 × 10- 3mol / L,respectively. The detection limit was 4. 9 × 10-7mol / L. The proposed method was applied in the determination of AA in commercial orange juice,and 2,6-dichlorophenol indophenol titration method was used for verification. The results showed that the method was rapid,accurate and inexpensive,and was suitable for the determination of AA in orange juice and other food samples.

关 键 词：分子印迹 抗坏血酸 电聚合 电化学传感器 聚邻苯二胺 

分 类 号：O657.1[理学—分析化学] Q564[理学—化学]