充气糖果中胭脂红酸的高效液相色谱测定  

Determination of Carminic Acid in Aerated Confections by High Performance Liquid Chromatography

作　　者：张飞[1] 郑家概[1] 付强[1] 刘军[1] 李圣男[1] 林钦恒[1] 

机构地区：[1]中国广州分析测试中心,广东广州510070

出　　处：《广州化工》2013年第24期110-111,134,共3页GuangZhou Chemical Industry

摘　　要：建立充气糖果中胭脂红酸的高效液相色谱方法。糖果经乙醇／氨杉水超声提取，水浴蒸至近干，氨水溶液定容。采用C18柱（4．6mm×250mm，5μm），流动相为0．01％磷酸溶液和甲醇／乙腈，梯度洗脱，流速1．0mL/min，检测波长494nm。胭脂红酸在2．5—40．0mg／L线性良好，回收率为87％～102％，RSD为1．75％，检出限为0．3mg／kg。该方法操作简便、准确，适于充气糖果中胭脂红酸的测定。A method for determination of carminic acid in aerated confections by high performance liquid chromatography （HPLC） was developed. The aerated confections were extracted by ultrasonic technique with ethanol/ ammonia/water. The extract evaporated nearly to dryness on water bath then volumed it by ammonia. HPLC separation of carminic acid was conducted on a C18 column （4. 6 mm ×250 mm, 5 μm） with a mobile phase of 0. 01% phosphoric acid and methanol/acetonitrile under a gradient elution condition at a flow rate of 1.0 mL/min and the detection was carried out at a wavelength of 494 nm. The linearity was obtained in the range of 2. 5 ~ 40. 0 mg/L. The average recoveries of samples were in the range of 87 - 102% and RSD was 1.75%. The determination of limit was 0.3 mg/kg. This method was found to be fast, reliable and good linearity with low relative standard deviation, which can be used for determination of earminic acid in aerated confections.

关 键 词：胭脂红酸 充气糖果 高效液相色谱法 

分 类 号：O657.7[理学—分析化学]