反应萃取法分离水中L-色氨酸和L-赖氨酸  

Separation of L-tryptophan and L-lysine in Aqueous Solution with Reactive Extraction

作　　者：杨新妮[1] 张剑[1] 杨平[1] 

机构地区：[1]武汉工业学院化学与环境工程学院,武汉430023

出　　处：《中国调味品》2014年第1期67-70,共4页China Condiment

基　　金：湖北省教育厅重点科研项目(D20121806)

摘　　要：以分光光度计为检测手段,通过对二甲氨基苯甲醛法和茚三酮法联用,建立了一种测定L-赖氨酸和L-色氨酸混合水溶液中两种氨基酸的含量的方法。以二(2-乙基己基)磷酸为萃取剂、正辛醇为溶剂构成的溶剂萃取体系,研究了水中L-色氨酸和L-赖氨酸在有机相中萃取分配行为。结果表明:在pH为2.0时,水相中的L-赖氨酸经有机相两次萃取后可得到纯品,平均回收率为77.2%。负载于有机相中的氨基酸,用等体积的1mol/L HCl反萃,再调反萃液酸度至pH为2.0,重复上述操作一次,可得色氨酸纯品,其平均回收率为76.0%。The determination method of L-lysine and L-tryptophan in the mixture of two amino acids has been established with p-dimethylaminobenzaldehyde method and ninhydrin method by using visible speetrophotometer. The extraetion distribution behaviors of L-tryptophan and L-lysine in organic phase are studied by using di （2-ethylhexyl） phosphoric acid as extractant and n-octyl alcohol as sol- vent. Pure L-lysine could be obtained by twice extraction of organic phase to aqueous solution at an acidity of pH 2.0 and its average recovery is 77.2%. After the amino acids loaded in organic phase are stripped into aqueous phase with equal volume of 1 mol/L HCl solution, the acidity of the aqueous solution is adjusted to pH 2.0. Subsequent operation repeated once as above, pure L-tryptophan could be obtained and its average recovery is 76.0%.

关 键 词：L-色氨酸 L-赖氨酸 二(2-乙基己基)磷酸 溶剂萃取 分离纯化 

分 类 号：TS201.24[轻工技术与工程—食品科学]