bcl-2硫代反义寡核苷酸提高胃癌细胞凋亡的敏感性  

Bcl-2 anti sense oligonucleotide sensitizes Fas mediated apoptosis of gastric cancer cells

作　　者：曹健 乔颖 闵军[2] 

机构地区：[1]广东省台山市人民医院外科,529200 [2]中山医科大学孙逸仙纪念医院外科

出　　处：《中华肿瘤杂志》2000年第6期466-468,共3页Chinese Journal of Oncology

摘　　要：目的　观察硫代反义寡核苷酸抑制bcl 2表达前后胃癌细胞对Fas介导凋亡的敏感性。方法　将bcl 2硫代反义寡核苷酸 (bcl 2SASON)转染胃癌细胞系MKN45 ,Western印迹法检测bcl 2蛋白表达 ;再应用激活型Fas抗体处理癌细胞 ,并用TUNEL染色 ,流式细胞仪检测凋亡 ,分析bcl 2表达与Fas诱导凋亡的关系。结果　bcl 2SASON转染后MKN45细胞bcl 2表达明显受到抑制 ,而且在经激活型Fas抗体处理后细胞凋亡指数为 (5 5 6± 4 7) % ,而未用bcl 2SASON处理的对照组则为 (8 4±2 1) % ,差异有显著性 (P <0 0 1)。结论　bcl 2表达的胃癌细胞能耐受Fas系统介导的细胞凋亡 ,用反义寡核苷酸下调bcl 2表达后 。Objective To study the effect of bcl 2 anti sense oligonucleotide on the sensitivity of gastric cancer cells to Fas mediated apoptosis.Methods Gastric cancer cell line MKN45 was transfected with bcl 2 anti sense oligonucleotide, and expression of bcl 2 was examined by Western blotting. Agonistic Fas antibody was used to induce apoptosis detected by TUNEL staining and flow cytometry.Results Bcl 2 expression in MKN45 cells transfected with bcl 2 anti sense oligonucleotide was markedly inhibited. When cultured with antibody apoptosis index of the anti sense oligonucleotide treated MKN45 cells was 55.6%±4.7% ( n =5), which was significantly higher than that of the control (8.4%±2.1%, n =5).Conclusion Expression of bcl 2 in gastric cancer cells may antagonize Fas mediated apoptosis.

关 键 词：胃肿瘤 BCL-2基因 反义寡核苷酸 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]