MUC-1基因在非小细胞肺癌的表达及其意义  

EXPRESSION AND SIGNIFICANCE OF MUC-1 GENE IN NON SMALL CELL LUNG CANCER

作　　者：冯久贤[1] 包国良[1] 时梅萍[1] 沙惠芳[1] 董强刚[1] 张立新[2] 孙丽亚[2] 李春海[2] 

机构地区：[1]上海市胸科医院,上海200030 [2]军事医学科学院,北京100850

出　　处：《肿瘤研究与临床》2000年第6期391-392,共2页Cancer Research and Clinic

摘　　要：目的 :检测非小细胞肺癌 MUC- 1的表达情况及其与临床分期和转移的相关性。方法 :应用反转录聚合酶链反应 (RT- PCR)和免疫组织化学方法在原发癌灶和转移淋巴结中检测 MU C- 1基因的表达水平。结果 :在5 6例非小细胞肺癌病例中 MU C- 1m RNA阳性表达率为 48.2 % ,MU C- 1蛋白阳性表达率为 5 3.6 %。临床分期中 期和 期患者阳性表达率 :MU C- 1m RNA为 33.3% ,MU C- 1蛋白为 40 % ,而 期患者则分别高达 6 5 .4%和6 9.2 % ,二者差异有显著性 (P<0 .0 5 )。原发癌灶和转移淋巴结的癌细胞 MUC- 1的染色强度可以从“+”增强到“+ +”,转移的癌细胞 MUC- 1高表达。结论 :MU C-Objective:To study the relationship between expression of MUC 1 gene and clinical classification in non small cell lung cancer(NSCLC).Methods:RT PCR and immunohistochemistry was used to study the expression of MUC 1 gene.Results:The positive expression rate of MUC 1 mRNA was 48.2%,and the positive expression rate of MUC 1 protein was 53.6% in NSCLC. In clinic classification with Ⅰ and Ⅱ degree,the positive expression rate of MUC 1 mRNA was 33.3%;but MUC 1 protein was 40% in degree Ⅲ were 65.4% and 69.2% respectively.There was a correlation between expression of MUC 1 and clinic classification.Conclusion:MUC 1 is an important tumor marker on the malignant potential and metastasis of NSCLC..

关 键 词：MUC-1 基因表达 肺肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]