QuEchERs-GC法测定蜜蜂生物体的氟胺氰菊酯残留  

Detection of fluvalinate residues in bee by QuEchERs-GC

作　　者：陈芳[1,2] 李熠[1,2] 王勇[1] 曹葳蕤 魏月[1] 吴黎明[1,2] 

机构地区：[1]中国农业科学院蜜蜂研究所 [2]农业部蜂产品质量安全风险评估实验室

出　　处：《中国蜂业》2014年第2期44-46,共3页Apiculture of China

基　　金：青年自然科学基金项目(31101771);国家蜂产业技术体系建设专项资金(NYCYTX-43)

摘　　要：本文旨在开发一种快速、高效且准确的蜜蜂生物体氟胺氰菊酯药物残留检测方法。研究了3种填料对蜜蜂样品的净化效果，实验结果表明：采用PSA和c-18两种填料既能够有效去除蜜蜂基质干扰且能够实现准确回收。标准曲线在0．010。1．0mg／L的浓度范围内，相关系数为0．9964。方法的检出限为4．52μg／kg，定量限为15．1μg／kg。三个不同浓度水平的平均回收率为78．4～96．7％，日内相对标准偏差均低于10％，而日间的相对标准偏差仅在20μg／kg加标浓度时超出10％。实际样品的测定表明，蜜蜂体内的氟胺氰菊酯残留峰值主要集中在施药后的3～7天，之后残留水平急剧下降至检出限附近。A quick, efficient and accuracy method to detect the fluvalinate residues in bee was developed. 3 kinds of QuEchERs tubes were chosen to pre-process bee samples.The results showed the combination of PSA and C-18 not only could remove the interference of substrate, but also get satisfactory recovery. In the range of 0.01-1mg/L, the correlation coefficient of standard curve was 0.9964; the lowest limit detection was 4.52μg/kg; the limit of quantitation was 15.1μg/kg. the average recovery of added samples in different levels ranged from 78.4% to 96.7%, and the R.S.D value in intra- and inter-day assays were belowd 10%, except for near the level of limit quantitation, which showed good precision and accuracy. The peak value of fluvalinate residues in bee sample arose in 3 to 7 days after using fluvalinate strips then the value declined sharply to near the value of lowest limit detection.

关 键 词：蜜蜂 氟胺氰菊酯 杀螨剂 QuEchERs法残留 

分 类 号：S859.84[农业科学—临床兽医学]