牛磺酸对大鼠缺血心肌血管新生作用的研究  

Effects of taurine on ischemia-reperfusion myocardium angiogenesis in rats

作　　者：王亚霓[1] 刘欣[1] 张颖[2] 张曦[2] 王意涵[3] 徐哲龙[1] 

机构地区：[1]天津医科大学病理生理学教研室,天津300070 [2]天津医科大学药理学教研室,天津300070 [3]天津医科大学第二医院泌尿外科,天津300211

出　　处：《天津医科大学学报》2014年第1期4-7,F0003,共5页Journal of Tianjin Medical University

基　　金：国家自然科学基金资助项目(81200534)

摘　　要：目的:研究牛磺酸对大鼠缺血心肌血管新生的作用并探讨其作用机制。方法:选用健康雄性Wistar大鼠80只,随机分为4组,每组20只:缺血再灌注模型组,低、中、高剂量牛磺酸处理组。缺血再灌注模型组口服生理盐水10 mL/kg,每日1次,连续7 d。低、中、高剂量牛磺酸处理组牛磺酸200、400、800 mg/kg,每日1次,口服给药,连续7 d。采用大鼠心脏冠状动脉缺血再灌注的方法,连续监测心电图,观察牛磺酸对大鼠心肌缺血再灌注后心律失常、心肌梗死范围的影响,并观察牛磺酸对血清中血管内皮生长因子(VEGF)、低氧诱导因子-α(HIF-α)、血小板-内皮细胞粘附分子(CD34)以及心肌组织中VEGF含量的影响。结果:与对照组比较,随着牛磺酸剂量的增高可明显降低动物室性早搏(VPC)和室性心动过速(VT)的发生率,心肌梗死范围也显著减小,同时在光镜下观察各组随牛磺酸剂量的递增可促进心肌间的血管新生。牛磺酸高、中、低剂量组大鼠血清中VEGF、HIF-α、CD34与心肌中的VEGF活性明显升高(P<0.01)。结论:牛磺酸对大鼠缺血心肌血管新生具有促进作用。Objective： To study the effects of taurine on ischemia-reperfusion （I/R） myocardium angiogenesis in rats. Methods： Eighty healthy male Wistar rats were randomly divided into four groups, with n=20 in each group： The I/R group and the taurine of 3 dosages groups. The I/R group was directly given normal saline 10 mL/kg with oral administration once a day for one week. Each of taurine group was given oral administration 200 mg/kg, 400 mg/kg, 800 mg/kg, respectively, once a day for one week. The model of myocardial I/R injury was established in all of the groups. Blood pressure and ECG were obtained. Myocardial infarct size was examined. Activities of VEGF, HIF-a and CD34 were detected. Results： Compared with the control group, taurine significantly decreased the incidence of ventricular premature contraction （VPC） and ventricular tachycardia （VT） in rats during ischemia,and the myocardial infarction was alleviated in each taurine group in dosage-dependent manner. The levels of VEGF, HIF-a, CD34 and myoeardium level of VEGF significantly increased, and infarct size of myocardial was significantly reduced. Conclusion： The myocardial angiogenesis may be involved in the mechanism of taurine in protecting myocardium against I/R injury.

关 键 词：牛磺酸 心肌新生血管 缺血 再灌注 血小板-内皮细胞粘附分子 血管内皮生长因子 大鼠 

分 类 号：R363.13[医药卫生—病理学]