4种海产资源中烷氧基甘油的气相色谱-质谱分析  

Gas chromatograph-mass spectrum analysis of alkoxyglycerols of four marine resources

作　　者：郭正霞[1] 王静凤[1] 楼乔明[2] 孙兆敏[1] 薛长湖[1] 徐杰[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]宁波大学海洋学院,浙江宁波315211

出　　处：《中国海洋药物》2014年第1期17-22,共6页Chinese Journal of Marine Drugs

基　　金：海洋公益性行业科研专项(201105029);国际科技合作项目(2010DFA31330);"泰山学者"建设工程专项经费;长江学者和创新团队发展计划资助;山东省科技发展计划(2012G0021506)资助

摘　　要：目的分析烷氧基甘油(alkoxyglycerols,AKGs)硅烷化衍生物的裂解规律和质谱特征;采用气相色谱-质谱(gas chromatograph-mass spectrum,GC-MS)联用技术分析比较鲨鱼肝油、海鳗鱼油、金枪鱼油和海星脂质4种海产资源中的AKGs组成。方法将4种海产资源中的烷氧基甘油酯(Di-esterified AKGs,DEAKGs)水解为游离态AKGs,并采用硅烷化衍生结合GC-MS联用技术对其AKGs组成进行分析。结果确定基峰离子m/z205和[M-15]+可作为判断烷氧基甘油硅烷化衍生物的重要依据。从4种海产资源中共鉴定出14种烷氧基甘油,以C16∶0-AKG、C18∶0-AKG和C18∶1-AKG为主,其中鲨鱼肝油中C18∶1-AKG含量最高,为43.76%;海鳗鱼油和金枪鱼油中C16∶0-AKG含量最高,分别为44.35%和31.31%;而海星脂质中C18∶0-AKG含量最高,为48.94%。结论基峰离子m/z205和[M-15]+可作为判定烷氧基甘油硅烷化衍生物的依据;4种海产资源中烷氧基甘油以C16∶0-AKG、C18∶0-AKG和C18∶1-AKG为主,但在AKGs的具体组成上存在较大差异。Objective To analyze the dissociation regularity and mass spectrum characterization of alkox- yglycerols （AKGs） after silylation derivatization and investigate the AKGs from the oil of shark liver. eel,tuna and star fish using gas chromatograph-mass spectrum（GC-MS）. Methods The Di-esterified AKGs （DEAKGs） from the four marine resources were hydrolyzed into their free forms, and silylation derivatization with GC-MS was utilized to determine their composition of AKGs. Results Base peak ion m/z205 and [M-15]^＋ could be used as the important basis of the silanization derivatives of AKGs. Fourteen AKGs were determined from the four marine resources, among which C16 ： 0-AKG.C18 ： 0- AKG and C18 ： 1-AKG were the main components. C18 -AKG was the main component from shark liver oil with a content of 43.76%, while C16 ： 0-AKG was the main component from tuna oil and eel oil with a content of 31.31% and 44. 35% respectively, and for star fish, C18 ： 0-AKG was the main component with a content of 48. 94%. Conclusion Base peak ion m/z205 and [M-15]^＋ are the qualitative basis of the silanization derivatives of AKGs. C16 ： 0-AKG. C18 ： 0-AKG and C18 ： 1-AKG are the main components from the four marine resources, but there are large differences in specific composition of AKGs.

关 键 词：海产资源 烷氧基甘油 硅烷化衍生 气相色谱-质谱法 

分 类 号：R917[医药卫生—药物分析学]