Chemical constituents of Euphorbia tibetica and their biological activities  

作　　者：YANG Da-Song HE Qiu-Xia YANG Yong-Ping LIU Ke-Chun LI Xiao-Li 

机构地区：[1]Key Laboratory of Economic Plants and Biotechnology [2]Germplasm Bank of Wild Species in Southwest China [3]Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences [4]Biology Institute of Shandong Academy of Sciences

出　　处：《Chinese Journal of Natural Medicines》2014年第1期38-42,共5页中国天然药物（英文版）

基　　金：supported by National Natural Science Foundation of China(No.31300293);General Project of Applied Foundation Research,Yunnan Province(No.2013FB067);Basic Research Project of Ministry of Science and Technology of China(No.2012FY110300);Major State Basic Research Development Program(No.2010CB951704)

摘　　要：AIM: To investigate the chemical constituents and their biological activities of the aerial parts of Euphorbia tibetica. METHOD: Compounds were isolated and purified by various chromatographic methods, and their structures were elucidated through the use of extensive spectroscopic techniques including 2D-NMR, the structures of compounds 5 and 6 were confirmed by single-crystal X-ray crystallography. Bioactivities of all the isolated compounds were evaluated by the MTT method on A549 and anti-angiogenesis assay. RESULTS: One new compound, methyl 4-epi-shikimate-3-O-gallate(1), together with twenty-three known constituents(2–24) were isolated from the aerial parts of E. tibetica. CONCLUSION: Compound 1 is new, and the other compounds were isolated from this plant for the first time. Compounds 5–7, 9, 11, 17, 18 and 20 exhibited inhibitory effects on the growth of human lung cancer cell A549 and compounds 5, 7, 12, 13, 17 and 19 showed anti-angiogenic effects in a zebrafish model.AIM： To investigate the chemical constituents and their biological activities of the aerial parts of Euphorbia tibetica. METHOD： Compounds were isolated and purified by various chromatographic methods, and their structures were elucidated through the use of extensive spectroscopic techniques including 2D-NMR, the structures of compounds 5 and 6 were confirmed by single-crystal X-ray crystallography. Bioactivities of all the isolated compounds were evaluated by the MTT method on A549 and anti-angiogenesis assay. RESULTS： One new compound, methyl 4-epi-shikimate-3-O-gallate （1）, together with twenty-three known constituents （2-24） were isolated from the aerial parts ofE. tibetica. CONCLUSION： Compound 1 is new, and the other compounds were isolated from this plant for the first time. Compounds 5-7, 9, 11, 17, 18 and 20 exhibited inhibitory effects on the growth of human lung cancer cell A549 and compounds 5, 7, 12, 13, 17 and 19 showed anti-angiogenic effects in a zebrafish model.

关 键 词：Euphorbia tibetica EUPHORBIACEAE Shikimate derivatives DITERPENES CYTOTOXICITY 

分 类 号：R284.1[医药卫生—中药学]