新疆双峰驼乳脂肪酸化学成分的GC/MS分析  

Analysis of comparison of fatty acids in camel milk from Xinjiang by gas chromatography—mass spectrometry

作　　者：王学清[1] 蒋新月[1] 杨洁[1] 

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830046

出　　处：《中国乳品工业》2014年第1期11-12,17,共3页China Dairy Industry

基　　金：新疆维吾尔自治区自然科学基金(2010211A04)

摘　　要：对新疆驼乳中脂肪酸的组成和质量分数进行测定。采用哥特里-罗兹法提取乳中的脂肪，氢氧化钾-甲醇溶液法甲酯化，并采用气相色谱-质谱联用技术进行了分离鉴定。分离出35种成分，共确认了其中33种成分。采用峰面积归-化法确定了各成分的相对质量分数。结果表明：驼乳脂肪酸以中、长链为主，中链脂肪酸（C12-C16）相对质量分数在50％以上，长链脂肪酸（C18-C24）约占47％以上；饱和脂肪酸以棕榈酸、豆蔻酸和硬脂酸为主，质量分数占到总量的60．35％；不饱和脂肪酸以油酸为主，质量分数占39．65％，饱和脂肪酸与不饱和脂肪酸比为1．52：1．在新疆双峰驼乳中还存在质量分数约为13．34％的奇数碳脂肪酸。To determine the composition and mass fraction of fatty acids in Xinjiang camel milk, the fatty oils of camel milk were extracted by Rose-Gettlieb method and methyl-esterified by the method of potassium hydroxide-methanol solution, then analyzed by gas chromatogra- phy-mass spectrometry （GC/MS）. 35 constituents were separated and 33 compounds were identified, the relative mass fraction of each com- ponent was determined by peak area normalization method. The results indicated that the major fatty acid are medium and long chain fatty acids, the content of medium-chain fatty acids （C12 - C16） is more than 50% , and long chain fatty acids （C18 - C24） accounted for more than 47%. Palmitic aci, nutmeg acid and stearic acid are major saturate fatty acid, and the content reached 60.35%. Oleic acid is major unsaturated fatty acid and the content reached 39.65%. The ratio of saturated fatty acids and unsaturated fatty acids is 1.52：1. The odd number carbon fatty acids are also contained about 13.34% in xinjiang bactrian camel milk.

关 键 词：驼乳 脂肪酸 气相色谱-质谱联用(GC MS) 

分 类 号：S824[农业科学—畜牧学]