褐藻素通过内质网途径诱导人乳腺癌MCF-7细胞凋亡的实验研究  

Fucoxanthin Induces Human MCF-7 Breast Cancer Cells Apoptosis via Endoplasmic Reticulum Pathway

作　　者：侯莉莉[1] 许秋菊[1] 胡国强[1] 谢松强[1] 

机构地区：[1]河南大学化学生物学研究所,河南开封475004

出　　处：《中国药学杂志》2014年第2期117-120,共4页Chinese Pharmaceutical Journal

基　　金：河南省基础与前沿技术研究项目(No102300410095);河南省高校青年骨干教师资助计划(No2009GGJS-022)

摘　　要：目的探讨褐藻素诱导人乳腺癌MCF-7细胞凋亡及其分子机制。方法以四甲基偶氮唑蓝法检测细胞活力,流式细胞仪检测细胞凋亡率、细胞周期及细胞内游离钙的变化,蛋白质印迹法检测凋亡相关蛋白caspase-4、caspase-7、caspase-9、Bcl-2、Bax、细胞色素C的表达。结果褐藻素剂量依赖性的抑制乳腺癌MCF-7细胞的增殖、升高细胞内游离钙含量,阻滞细胞周期于G0/G1期并诱导其凋亡;上调Bax并下调Bcl-2;诱导caspase-4、caspase-7、caspase-9、钙蛋白酶的活化及细胞色素C的释放。结论褐藻素通过Ca2+/钙蛋白酶/caspase-4途径诱导人乳腺癌MCF-7细胞凋亡。OBJECTIVE The antiproliferative effects and molecular mechanism of fucoxanthin were evaluated in human MCF-7 breast cancer cells. METHODS Cell viability was assessed by MTT assay; cell cycle distribution, apoptosis and [ Ca2＋ I i were measured by FACScan; the protein expression of caspase-4, caspase-7, caspase-9, Bcl-2, Bax, calpain and cytochrome C in the MCF- 7 cells was evaluated by Western Blotting. RESULTS Fucoxanthiu exerted potent antiproliferative effects, induced [ Ca2＋ ] ~ overload and apoptosis in MCF-7 ceils. Furthermore, fucoxanthin-mediated apoptosis was related with the activation of caspase-4, caspase-7, caspase-9, cytochrome C release from mitochondrion, up-regulation of Bax and calpain, and also down-regulation Bcl-2 in a dose-de- pendent manner. CONCLUSION Fucoxanthin can induce MCF-7 ceils apoptosis via Ca2＋/calpain/caspase-4 pathway.

关 键 词：褐藻素 凋亡 内质网 CA2+ 钙蛋白酶 CASPASE 

分 类 号：R965[医药卫生—药理学]