发酵罐水平的气提-萃取-发酵生产丁醇  

Gas Stripping-Extraction-Fermentation in Fermentor for Butanol Production

作　　者：王鑫昕[1] 张延平[2] 

机构地区：[1]邯郸学院生物科学系,河北邯郸056005 [2]中国科学院微生物研究所,北京100101

出　　处：《沈阳农业大学学报》2013年第6期823-826,共4页Journal of Shenyang Agricultural University

基　　金：国家高技术研究发展计划(863计划)项目(2006AA02Z237)

摘　　要：在优化的萃取-发酵工艺基础上,研究补料、连续气提(氮气流速0.3L·min-1)对丁醇发酵的影响。结果表明:在7L发酵罐中,于传统丙酮丁醇发酵24h时,将油醇和癸醇按4∶1(v/v)混匀组成有机溶剂,按有机溶剂和培养基1∶5(v/v)加入到发酵液上方,并于24h从发酵罐底部持续通入惰性气体氮气,进行连续气提直至静置培养72h停止,有利于丁醇发酵,该气提-萃取-发酵工艺条件下,丁醇产量达16.39g·L-1,总溶剂产量达24.40g·L-1,比传统发酵分别提高了51.5%和35.7%;丁醇和总溶剂生产强度比传统发酵分别提高51.5%和35.7%,对丁醇的基质转化率提高了16.08%。Based on the optimization of the extraction and fermentation, the effect of feeding and continuous gas stripping （flow rate of 0.3L nitrogen per rain） on fermentation was researched. In 7L fermentor, oleyl alcohol and decanol alcohol with the volume ratio of 4：1 as the organic solvent was added above on fermentation broth at 24h based on classical butanol fermentation, according to the organic solvent and the medium volume ratio 1：5 （extraction conditions）. Then nitrogen gas was continuously brought in from the bottom of fermentor at 24h with continuous gas stripping till 72h. The gas stripping--extraction-fermentation process above was conducive to butanol fermentation, for butanol and solvent production were respectively 16.39g·L^-1 and 24.40g·L^-1, 51.5% and 35.7% higher than the classical butanol fermentation in fermentor. Butanol and solvent productivities by gas stripping-extraction-fermentation respectively increased by 51.5% and 35.7% compared with traditional fermentation, and conversion rate of raw material to butanol increased by 16.08%.

关 键 词：丁醇 总溶剂 气提-萃取-发酵 发酵 丙酮丁醇梭菌 

分 类 号：Q815[生物学—生物工程]